Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D0856_RS17485 Genome accession   NZ_CP033144
Coordinates   3602865..3604613 (+) Length   582 a.a.
NCBI ID   WP_039988233.1    Uniprot ID   -
Organism   Vibrio owensii strain V180403     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3597865..3609613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0856_RS17465 (D0856_17465) pilM 3600162..3601178 (+) 1017 WP_045412016.1 type IV pilus assembly protein PilM Machinery gene
  D0856_RS17470 (D0856_17470) - 3601162..3601739 (+) 578 Protein_3206 PilN domain-containing protein -
  D0856_RS17475 (D0856_17475) pilO 3601732..3602325 (+) 594 WP_009705519.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0856_RS17480 (D0856_17480) pilP 3602315..3602830 (+) 516 WP_038896738.1 pilus assembly protein PilP Machinery gene
  D0856_RS17485 (D0856_17485) pilQ 3602865..3604613 (+) 1749 WP_039988233.1 type IV pilus secretin PilQ family protein Machinery gene
  D0856_RS17490 (D0856_17490) aroK 3604803..3605321 (+) 519 WP_005438434.1 shikimate kinase AroK -
  D0856_RS17495 (D0856_17495) aroB 3605353..3606453 (+) 1101 WP_122068744.1 3-dehydroquinate synthase -
  D0856_RS17500 (D0856_17500) - 3606507..3608000 (+) 1494 WP_122068746.1 AAA family ATPase -
  D0856_RS17505 (D0856_17505) - 3608080..3608889 (+) 810 WP_025522125.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  D0856_RS17510 (D0856_17510) - 3608906..3609562 (+) 657 WP_122068748.1 site-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63891.99 Da        Isoelectric Point: 4.5148

>NTDB_id=321846 D0856_RS17485 WP_039988233.1 3602865..3604613(+) (pilQ) [Vibrio owensii strain V180403]
MKMRQGLTLTPRKLAGIFSLWFFLLLAPFVAAQEVKSNSLKTIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESIEIFRETSTTRLVAYIDEEYTHDYRLTGRYLEIKVGKIKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGEDGGSSVGIDDFLNVNLAATSPNA
TSLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=321846 D0856_RS17485 WP_039988233.1 3602865..3604613(+) (pilQ) [Vibrio owensii strain V180403]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAGCAGGGATCTTCTCTTTGTGGTTCTTTTTACTTTTAGC
CCCTTTTGTAGCAGCACAGGAAGTAAAGTCTAACTCGCTAAAGACCATCGACTTTCGCACAAACAAAGACAAAGATGCCG
TGATTGTTGTAGAACTTGCGTCCCCTGCAGCAATCGTTGATATCAAGCGCGTACAGGAAGGCTTGAGTATCGACTTACTC
AACACATCTGTAAAAGACGACCAGCTTTACCTTCTGGATGTAAAAGACTTTTCTACGGTCGTCGAGAGTATTGAAATATT
CCGAGAGACCTCAACTACACGTTTAGTTGCTTATATTGATGAGGAATACACTCACGATTACCGTCTGACTGGGCGTTACC
TAGAGATAAAAGTTGGTAAGATAAAAGCCAACGAAAAAGCACCGGATAAGAGCATTCTAGAAAAAGAAGGGAAGCTGATT
TCAATTAACTTCCAAGACATTCCTGTGCGAAACGTATTGCAACTCATCGCCGACTACAACCAATTTAACTTAGTGGTATC
CGACTCTGTTGAAGGCAACTTAACTTTGCGTCTTGATGGTGTGCCTTGGCAGCAAGTGCTCGATATCATTCTTCAAGTAA
AAGGCTTGGATAAGCGTGTTGATGGCAACGTGATTTTGGTGGCGCCGAAAGACGAACTGGATCTGCGTGAAAAGCAACAA
TTAGAGAAACAGAAGCTTGAGCAGGAGATGGGTGAGCTTTCTTCTGAGATCATCAAAGTTAACTTCGCTAAAGCGTCGGA
CATTGCTGAGATGATTAATGGCGAGGGTAATATCAGTATGCTCTCAGAACGTGGCAGCATGACCATTGATGAACGGACAA
ACTCACTCTTAATTAGAGAGCTACCAGAGAACATTGAGGTTATCCGAGAGATCATTGAGTCTTTGGATATTCCGGTCAAA
CAAGTTCAGATTGAAGCGCGAATCGTCACCATCAATGAAGGTAATATGGACGAACTTGGTGTGCGTTGGGGCTTTACCTC
TATCAACGGTAGCCACACTGTCGGTGGTTCGATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGATG
GCGAAGGTGAAGATGGTGGCAGCAGTGTTGGTATTGATGACTTCCTAAACGTGAACTTGGCTGCAACCAGCCCGAATGCG
ACCAGTCTTGCTTTCCAAGTTGCGAAGTTAGGCTCAGACACACTGCTTGATTTAGAGCTTTCTGCGCTGCAACAAGAATC
CAAGGCAGAGATTATTTCGAGCCCGCGATTGATTACCACTAACAAGAAGCCTGCTTATATCGAGCAAGGTACCGAAATCC
CGTACCTTGAGTCTTCTTCCAGTGGCGCAACGTCTGTCACGTTCAAAAAAGCCGTATTGAGCTTAAAAGTGACACCACAG
ATTACCCCTGACAATCGTTTAGTGTTGGATTTGAGCGTTACGCAAGATAGACCGGGTCAAGTTGTAAAAACAGGCACTGG
TGAAGCGGTCGCGATCGATACTCAACGCATTGGCACACAAGTTTTAGTCAATAACGGTGAAACGGTTGTGTTGGGTGGTA
TTTTTCAACATAGCATCACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTTCGT
CGAAGTTATGAAAATGTCGGCAAAAGTGAACTGTTGATTTTTGTCACGCCTAAAGTTGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

95.704

100

0.957
  pilQ Vibrio cholerae strain A1552

76.761

97.595

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.761

97.595

0.749
  pilQ Pseudomonas aeruginosa PAK

40.522

98.797

0.4
  pilQ Legionella pneumophila strain ERS1305867

41.176

96.392

0.397
  pilQ Legionella pneumophila strain Lp02

41.176

96.392

0.397
  pilQ Acinetobacter baumannii D1279779

36.851

100

0.378
  pilQ Acinetobacter baumannii strain A118

36.851

100

0.378
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369

Multiple sequence alignment