Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D0856_RS17465 Genome accession   NZ_CP033144
Coordinates   3600162..3601178 (+) Length   338 a.a.
NCBI ID   WP_045412016.1    Uniprot ID   -
Organism   Vibrio owensii strain V180403     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3595162..3606178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0856_RS17450 (D0856_17450) - 3595534..3596262 (-) 729 WP_005438421.1 redoxin family protein -
  D0856_RS17455 (D0856_17455) oxyR 3596438..3597346 (+) 909 WP_033005806.1 DNA-binding transcriptional regulator OxyR -
  D0856_RS17460 (D0856_17460) - 3597413..3599983 (-) 2571 WP_041061315.1 PBP1A family penicillin-binding protein -
  D0856_RS17465 (D0856_17465) pilM 3600162..3601178 (+) 1017 WP_045412016.1 type IV pilus assembly protein PilM Machinery gene
  D0856_RS17470 (D0856_17470) - 3601162..3601739 (+) 578 Protein_3206 PilN domain-containing protein -
  D0856_RS17475 (D0856_17475) pilO 3601732..3602325 (+) 594 WP_009705519.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0856_RS17480 (D0856_17480) pilP 3602315..3602830 (+) 516 WP_038896738.1 pilus assembly protein PilP Machinery gene
  D0856_RS17485 (D0856_17485) pilQ 3602865..3604613 (+) 1749 WP_039988233.1 type IV pilus secretin PilQ family protein Machinery gene
  D0856_RS17490 (D0856_17490) aroK 3604803..3605321 (+) 519 WP_005438434.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38002.31 Da        Isoelectric Point: 6.0152

>NTDB_id=321843 D0856_RS17465 WP_045412016.1 3600162..3601178(+) (pilM) [Vibrio owensii strain V180403]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKERIVAENNTINHQEIVKTLKEMRKELPIRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVMQAFAHQSPFPVEELSLDFVKVDTDEQMGGTESYQVFATRREVVESRVETLTKSGLKTV
LVDVHSQSLGHIWKLTAERFPEKNKHCLLDIGAMSSSFTMFTEQGELFHKEFPCGTRTTNDLSQHDPLSDDIQTATEEFN
RQMVERVKRQMQLYTSFNGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLSLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDDAASH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=321843 D0856_RS17465 WP_045412016.1 3600162..3601178(+) (pilM) [Vibrio owensii strain V180403]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCTTTATTGGGTTACAAAGAAATAATACTTAAGGAACGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAGAAAAGAGCTCCCAATTCGAGCTAGAAAGGTCGCAATTTCGGTTCCAGACAATGCC
GTGATCAGCAAAAAGCTACAAATAGAGCAGAACCTAGAAGAAAGCGAAAAGGAATTTGCGGTGATGCAAGCCTTTGCGCA
TCAGTCTCCCTTTCCGGTTGAAGAGCTCAGCCTCGACTTTGTAAAGGTTGATACGGATGAGCAGATGGGCGGCACAGAGA
GCTACCAAGTGTTTGCAACACGCAGAGAGGTGGTGGAAAGCCGAGTGGAGACATTAACAAAGTCTGGCCTAAAAACCGTG
CTGGTGGATGTGCACTCACAAAGTCTTGGTCATATATGGAAGCTAACCGCAGAACGTTTTCCAGAAAAGAATAAGCACTG
CTTGTTGGACATTGGTGCGATGAGCAGCTCTTTCACTATGTTTACCGAGCAAGGAGAGTTATTCCATAAGGAGTTTCCTT
GCGGTACCCGCACAACAAACGATTTGTCTCAACACGATCCACTCAGTGACGATATTCAAACCGCAACTGAAGAGTTCAAT
CGACAAATGGTTGAACGTGTTAAGCGTCAGATGCAGCTCTACACCTCGTTCAATGGTAGCCAAGATATCAAAGGCATTTG
GCTTTCCGGAGAGGGGGCGGCTACGCCAATGTTAGCTGAGGAACTATCAAGTCAATTGGCACTAGAGTGTGAACTTCTCA
ATCCTCTTAGTTTGTTTGAAATGAAAGTCTCTAAACGTAAACGTCGAGCGGCAGATTGGCAGCACTTCACGGCAGCTGCA
GGTTTAGCGGTACGCGGTATTCAGTGGTTGGGAGGTGACGATGCTGCATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

89.053

100

0.891
  pilM Vibrio cholerae strain A1552

47.989

100

0.494

Multiple sequence alignment