Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D0871_RS02065 Genome accession   NZ_CP033141
Coordinates   396933..397949 (+) Length   338 a.a.
NCBI ID   WP_069514340.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 160807     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391933..402949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0871_RS02050 (D0871_02050) - 392357..393085 (-) 729 WP_015297397.1 redoxin family protein -
  D0871_RS02055 (D0871_02055) oxyR 393256..394164 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  D0871_RS02060 (D0871_02060) - 394220..396790 (-) 2571 WP_029815565.1 PBP1A family penicillin-binding protein -
  D0871_RS02065 (D0871_02065) pilM 396933..397949 (+) 1017 WP_069514340.1 type IV pilus assembly protein PilM Machinery gene
  D0871_RS02070 (D0871_02070) pilN 397933..398511 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  D0871_RS02075 (D0871_02075) pilO 398504..399097 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0871_RS02080 (D0871_02080) pilP 399087..399602 (+) 516 WP_025818422.1 pilus assembly protein PilP Machinery gene
  D0871_RS02085 (D0871_02085) pilQ 399634..401382 (+) 1749 WP_025818421.1 type IV pilus secretin PilQ family protein Machinery gene
  D0871_RS02090 (D0871_02090) aroK 401571..402089 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.33 Da        Isoelectric Point: 7.0224

>NTDB_id=321771 D0871_RS02065 WP_069514340.1 396933..397949(+) (pilM) [Vibrio parahaemolyticus strain 160807]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYKVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=321771 D0871_RS02065 WP_069514340.1 396933..397949(+) (pilM) [Vibrio parahaemolyticus strain 160807]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCAAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATAAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTATTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCTGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479

Multiple sequence alignment