Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D0812_RS01720 Genome accession   NZ_CP033137
Coordinates   348217..349233 (+) Length   338 a.a.
NCBI ID   WP_054824717.1    Uniprot ID   A0AAP9K8V9
Organism   Vibrio owensii strain 1700302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343217..354233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0812_RS01705 (D0812_01705) - 343589..344317 (-) 729 WP_054824719.1 redoxin family protein -
  D0812_RS01710 (D0812_01710) oxyR 344493..345401 (+) 909 WP_033005806.1 DNA-binding transcriptional regulator OxyR -
  D0812_RS01715 (D0812_01715) - 345468..348038 (-) 2571 WP_054824718.1 PBP1A family penicillin-binding protein -
  D0812_RS01720 (D0812_01720) pilM 348217..349233 (+) 1017 WP_054824717.1 type IV pilus assembly protein PilM Machinery gene
  D0812_RS01725 (D0812_01725) pilN 349217..349795 (+) 579 WP_054824716.1 PilN domain-containing protein Machinery gene
  D0812_RS01730 (D0812_01730) pilO 349788..350381 (+) 594 WP_009705519.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0812_RS01735 (D0812_01735) pilP 350371..350886 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  D0812_RS01740 (D0812_01740) pilQ 350921..352669 (+) 1749 WP_045412013.1 type IV pilus secretin PilQ family protein Machinery gene
  D0812_RS01745 (D0812_01745) aroK 352859..353377 (+) 519 WP_005438434.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37987.30 Da        Isoelectric Point: 6.1873

>NTDB_id=321724 D0812_RS01720 WP_054824717.1 348217..349233(+) (pilM) [Vibrio owensii strain 1700302]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKERIVAENNTINHQEIVKTLKEMRKELPIRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVMQAFAHQSPFPVEELSLDFVKVDTNEQMGGTESYQVFATRREVVESRVDTLTKSGLKTV
LVDVHSQSLGHIWKLTAERFPEKNKHCLLDIGAMSSSFTMFTEQGELFHKEFPCGTRTTNDLSQHDPLSDDIQTATEEFN
RQMVERVKRQMQLYTSFNGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLSLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDDAASH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=321724 D0812_RS01720 WP_054824717.1 348217..349233(+) (pilM) [Vibrio owensii strain 1700302]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCTTTATTGGGTTACAAAGAAATAATACTTAAGGAACGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAGAAAAGAGCTCCCAATTCGAGCTAGAAAGGTCGCAATTTCGGTTCCAGACAATGCT
GTGATCAGCAAAAAGCTACAAATAGAGCAGAACCTAGAAGAAAGCGAAAAGGAATTTGCGGTGATGCAAGCCTTTGCGCA
TCAATCTCCCTTTCCGGTTGAAGAGCTCAGCCTCGATTTTGTAAAGGTTGATACGAATGAGCAGATGGGCGGCACAGAGA
GCTACCAAGTGTTTGCAACACGCAGAGAGGTGGTGGAAAGCCGAGTGGATACATTAACGAAGTCTGGCCTAAAAACCGTG
CTGGTGGATGTGCACTCACAAAGTCTTGGTCATATATGGAAGCTAACCGCAGAACGTTTTCCTGAAAAGAATAAGCATTG
CTTGTTAGACATTGGTGCGATGAGTAGCTCTTTCACTATGTTTACCGAGCAAGGTGAGCTATTCCATAAGGAATTTCCTT
GCGGTACTCGCACAACAAACGATTTGTCTCAACACGATCCACTCAGTGACGATATCCAAACTGCAACTGAAGAATTCAAT
CGACAAATGGTTGAACGTGTTAAGCGTCAGATGCAGCTCTATACCTCGTTCAATGGTAGCCAAGATATCAAAGGTATTTG
GCTTTCCGGTGAGGGGGCGGCTACGCCAATGTTAGCTGAGGAACTATCGAGTCAATTGGCACTAGAGTGTGAACTTCTCA
ATCCTCTTAGTTTGTTTGAAATGAAAGTCTCTAAACGTAAACGCCGAGCGGCAGATTGGCAGCATTTCACCGCAGCTGCA
GGTTTAGCGGTACGCGGTATTCAGTGGTTGGGAGGTGACGATGCTGCATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

88.462

100

0.885
  pilM Vibrio cholerae strain A1552

47.839

100

0.491

Multiple sequence alignment