Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   D0784_RS14865 Genome accession   NZ_CP033134
Coordinates   3146014..3146589 (-) Length   191 a.a.
NCBI ID   WP_005432710.1    Uniprot ID   -
Organism   Vibrio campbellii strain 170502     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3141014..3151589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0784_RS14840 (D0784_14830) aroB 3141301..3142401 (-) 1101 WP_045380637.1 3-dehydroquinate synthase -
  D0784_RS14845 (D0784_14835) aroK 3142433..3142951 (-) 519 WP_010443904.1 shikimate kinase AroK -
  D0784_RS14850 (D0784_14840) pilQ 3143140..3144888 (-) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  D0784_RS14855 (D0784_14845) pilP 3144923..3145438 (-) 516 WP_045401529.1 pilus assembly protein PilP Machinery gene
  D0784_RS14860 (D0784_14850) pilO 3145428..3146021 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0784_RS14865 (D0784_14855) pilN 3146014..3146589 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  D0784_RS14870 (D0784_14860) pilM 3146573..3147589 (-) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene
  D0784_RS14875 (D0784_14865) - 3147768..3150335 (+) 2568 WP_122020084.1 penicillin-binding protein 1A -
  D0784_RS14880 (D0784_14870) oxyR 3150414..3151322 (-) 909 WP_012126437.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22194.53 Da        Isoelectric Point: 9.4267

>NTDB_id=321708 D0784_RS14865 WP_005432710.1 3146014..3146589(-) (pilN) [Vibrio campbellii strain 170502]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=321708 D0784_RS14865 WP_005432710.1 3146014..3146589(-) (pilN) [Vibrio campbellii strain 170502]
ATGCTGTATCACATTAACCTTCTTGCCTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCAATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTGGATTATCTCGCTCACTATATCTCTCAACTCGATAAACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAAGGGCTGCAAAATGGTCGCAATAAAACCACTGAGTTTATGAACCTGATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCCGCCAAGCTGCTGGATGTTGATATGCACTCCATTGTT
CATGGTAAAGCGCGCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTCATGTTTAAAGACGCCTCTATAAAGGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

99.476

100

0.995
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56

Multiple sequence alignment