Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D0784_RS14870 Genome accession   NZ_CP033134
Coordinates   3146573..3147589 (-) Length   338 a.a.
NCBI ID   WP_005432712.1    Uniprot ID   -
Organism   Vibrio campbellii strain 170502     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3141573..3152589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0784_RS14845 (D0784_14835) aroK 3142433..3142951 (-) 519 WP_010443904.1 shikimate kinase AroK -
  D0784_RS14850 (D0784_14840) pilQ 3143140..3144888 (-) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  D0784_RS14855 (D0784_14845) pilP 3144923..3145438 (-) 516 WP_045401529.1 pilus assembly protein PilP Machinery gene
  D0784_RS14860 (D0784_14850) pilO 3145428..3146021 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0784_RS14865 (D0784_14855) pilN 3146014..3146589 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  D0784_RS14870 (D0784_14860) pilM 3146573..3147589 (-) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene
  D0784_RS14875 (D0784_14865) - 3147768..3150335 (+) 2568 WP_122020084.1 penicillin-binding protein 1A -
  D0784_RS14880 (D0784_14870) oxyR 3150414..3151322 (-) 909 WP_012126437.1 DNA-binding transcriptional regulator OxyR -
  D0784_RS14885 (D0784_14875) - 3151497..3152225 (+) 729 WP_005432732.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37860.29 Da        Isoelectric Point: 6.3141

>NTDB_id=321709 D0784_RS14870 WP_005432712.1 3146573..3147589(-) (pilM) [Vibrio campbellii strain 170502]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=321709 D0784_RS14870 WP_005432712.1 3146573..3147589(-) (pilM) [Vibrio campbellii strain 170502]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCA
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5

Multiple sequence alignment