Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PCC8801_RS07835 Genome accession   NC_011726
Coordinates   1685453..1687474 (+) Length   673 a.a.
NCBI ID   WP_012594935.1    Uniprot ID   B7JVX3
Organism   Rippkaea orientalis PCC 8801     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680453..1692474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC8801_RS07815 (PCC8801_1606) - 1680990..1681415 (-) 426 WP_012594931.1 type II toxin-antitoxin system VapC family toxin -
  PCC8801_RS07820 (PCC8801_1607) - 1681425..1682228 (-) 804 WP_012594932.1 hypothetical protein -
  PCC8801_RS07825 (PCC8801_1608) - 1682397..1683674 (-) 1278 WP_012594933.1 serine hydrolase -
  PCC8801_RS07830 (PCC8801_1609) ald 1683801..1684892 (-) 1092 WP_012594934.1 alanine dehydrogenase -
  PCC8801_RS07835 (PCC8801_1610) pilB/pilB1 1685453..1687474 (+) 2022 WP_012594935.1 GspE/PulE family protein Machinery gene
  PCC8801_RS07840 (PCC8801_1611) pilT 1687504..1688616 (+) 1113 WP_041229755.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC8801_RS07845 (PCC8801_1612) - 1688717..1689931 (+) 1215 WP_012594937.1 type II secretion system F family protein -
  PCC8801_RS07850 (PCC8801_1613) - 1689939..1690511 (+) 573 WP_012594938.1 hypothetical protein -
  PCC8801_RS07855 (PCC8801_1614) - 1690590..1691549 (+) 960 WP_012594939.1 DUF362 domain-containing protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75236.01 Da        Isoelectric Point: 5.3304

>NTDB_id=32114 PCC8801_RS07835 WP_012594935.1 1685453..1687474(+) (pilB/pilB1) [Rippkaea orientalis PCC 8801]
MTYSSSTKRSRAVALRNYFSPFGNKLVETGYIAPDQMQQALVETRKTGLPLVEVVQSLTGRQLPPDLQRQYKKHHLFELK
ILYGVDSVDPEISPVADRQMAELIGTLISFDICRRHKLLPISKHEGDPPYILVAMVDPDNLAAQDDLNRIVRSKGLNLKR
MVITQEDYDRLLEQYHELQTELDQEKERLDKEKALEKLADISSLVDNMDNIGEVNDDDADSLDANEANNAPIINLVNKIL
AKALQEGVSDIHVEPQEESLRIRFRKDGVLQQAFDPLPKKIIAAVVARFKIMSDLDIAERRLPQDGKIRRIYQGRKVDFR
VNTLPSRYGEKVCLRILDNSATQLGLDKLITNQETLQIVRDLASRPFGLILVTGPTGSGKSTSLYSVLAERNNPGVNIST
AEDPIEYSLPGITQVQVIREKGMNFASILRAFMRQDPDVILVGETRDVETAKTAIEAALTGHLVLTTLHTNDAAGAIARL
DEMGVEPFMISGALLGVLAQRLMRRVCTECRVPYNPTKAELARFGLSASNQEEITIYKANKLTPEEITEAKTKGSLCAKC
NGSGYKGRVGVYEVMRNTEKIQSLINQGATTDRIKEAAVEEGMVTILAYSLQLVQEGYTTLEEVERVTFTDTGLESELKA
KRKSSLECQTCNAELQQEWLDCPYCLTPRFINN

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=32114 PCC8801_RS07835 WP_012594935.1 1685453..1687474(+) (pilB/pilB1) [Rippkaea orientalis PCC 8801]
ATGACTTATTCGTCTTCCACCAAGCGATCGCGTGCAGTCGCCCTGAGAAACTACTTCTCCCCCTTTGGGAATAAGTTGGT
TGAAACCGGATACATCGCACCCGATCAAATGCAGCAAGCCCTAGTTGAAACTCGTAAAACTGGACTTCCCCTTGTCGAAG
TGGTACAATCGCTAACCGGTCGTCAACTGCCCCCTGATTTACAACGACAATATAAAAAACACCATTTATTTGAACTAAAA
ATCCTCTATGGAGTAGATTCAGTCGATCCCGAAATTAGTCCCGTCGCGGATCGACAAATGGCCGAACTGATTGGCACTCT
AATCTCTTTTGACATCTGTCGTCGTCATAAACTGTTACCTATTTCTAAGCACGAAGGAGATCCCCCATATATCTTAGTGG
CTATGGTTGATCCCGACAACTTAGCCGCCCAAGACGATCTCAATCGCATTGTAAGATCTAAAGGACTCAACCTCAAACGC
ATGGTCATTACCCAAGAAGATTATGACCGACTCCTAGAACAATATCATGAATTGCAAACAGAACTAGACCAAGAAAAAGA
ACGTCTAGATAAAGAAAAAGCCCTGGAAAAACTGGCTGACATCTCCAGTTTAGTCGATAATATGGACAATATAGGGGAGG
TAAATGATGACGATGCCGATTCCCTAGATGCCAATGAAGCTAACAACGCCCCTATTATTAACCTAGTTAACAAAATTCTC
GCCAAAGCCCTACAAGAAGGGGTATCGGATATCCACGTCGAACCCCAAGAAGAATCCCTACGCATTCGTTTTCGTAAGGA
TGGGGTCTTACAACAAGCCTTTGATCCCCTGCCCAAAAAAATCATTGCAGCAGTGGTAGCACGGTTTAAGATTATGTCAG
ACTTAGACATTGCCGAACGCCGTCTGCCTCAAGATGGAAAAATCCGCCGCATCTACCAAGGACGCAAAGTAGACTTTCGG
GTGAATACCCTACCCAGTCGCTACGGCGAAAAAGTCTGTTTACGGATCTTAGATAACTCTGCTACCCAACTGGGATTAGA
TAAATTAATCACCAATCAAGAAACCCTACAAATTGTCCGCGACTTAGCCAGTCGTCCCTTTGGCTTAATTTTGGTAACAG
GACCGACGGGAAGTGGAAAATCCACCAGTTTGTACTCCGTGTTAGCCGAACGCAACAACCCAGGGGTTAACATTAGTACC
GCCGAAGATCCCATTGAATACTCCTTACCCGGCATTACCCAAGTCCAGGTGATTCGGGAAAAAGGCATGAACTTTGCTTC
TATTCTACGCGCCTTTATGCGACAAGATCCCGATGTCATTCTGGTGGGGGAAACGCGAGACGTAGAAACCGCTAAAACCG
CCATTGAAGCTGCCTTAACGGGTCACTTAGTCTTAACCACCCTCCATACTAACGATGCCGCCGGTGCGATCGCCCGTCTC
GATGAAATGGGAGTAGAACCCTTCATGATTTCTGGTGCCCTCCTCGGAGTCTTGGCGCAAAGGTTGATGCGACGAGTTTG
TACTGAGTGTCGGGTTCCCTATAACCCCACTAAAGCTGAACTCGCCCGTTTTGGTCTATCGGCCTCCAATCAAGAAGAAA
TCACTATCTACAAAGCCAATAAACTTACCCCAGAGGAAATTACTGAGGCTAAGACCAAGGGAAGCCTTTGTGCCAAATGT
AATGGTAGCGGTTATAAAGGACGGGTCGGGGTCTATGAAGTGATGCGCAATACCGAAAAGATTCAAAGTCTAATCAACCA
AGGGGCGACGACAGACCGCATTAAAGAAGCAGCCGTTGAAGAAGGGATGGTAACTATCCTTGCATACAGTTTGCAATTGG
TGCAAGAAGGCTATACCACCCTCGAAGAAGTGGAACGGGTGACGTTTACCGACACGGGACTCGAATCGGAATTAAAAGCA
AAACGCAAGAGTTCCCTCGAATGTCAAACCTGCAACGCCGAATTACAGCAAGAATGGTTGGACTGTCCCTATTGCCTCAC
CCCCAGATTTATTAACAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7JVX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

75.708

99.703

0.755

Multiple sequence alignment