Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   EST53_RS20460 Genome accession   NZ_CP040269
Coordinates   4202403..4203140 (-) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli strain 1500     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4197403..4208140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EST53_RS20430 (EST53_20430) yfiL 4197561..4197926 (-) 366 WP_000976004.1 DUF2799 domain-containing protein -
  EST53_RS20435 (EST53_20435) aroF 4198136..4199206 (+) 1071 WP_001168054.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  EST53_RS20440 (EST53_20440) tyrA 4199217..4200338 (+) 1122 WP_000225221.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  EST53_RS20445 (EST53_20445) pheA 4200381..4201541 (-) 1161 WP_000200116.1 bifunctional chorismate mutase/prephenate dehydratase -
  EST53_RS20450 (EST53_20450) pheL 4201640..4201687 (-) 48 WP_001386991.1 pheA operon leader peptide PheL -
  EST53_RS20455 (EST53_20455) raiA 4201791..4202132 (-) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  EST53_RS20460 (EST53_20460) comL 4202403..4203140 (-) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  EST53_RS20465 (EST53_20465) rluD 4203275..4204255 (+) 981 WP_000079107.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EST53_RS20470 (EST53_20470) yfiH 4204252..4204983 (+) 732 WP_000040115.1 purine nucleoside phosphorylase YfiH -
  EST53_RS20475 (EST53_20475) clpC 4205113..4207686 (+) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=320954 EST53_RS20460 WP_000197686.1 4202403..4203140(-) (comL) [Escherichia coli strain 1500]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=320954 EST53_RS20460 WP_000197686.1 4202403..4203140(-) (comL) [Escherichia coli strain 1500]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCAAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGAATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376