Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9777_RS03060 Genome accession   NZ_CP033054
Coordinates   580566..581003 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Bac57     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 575566..586003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9777_RS03035 - 576586..577722 (+) 1137 WP_021494202.1 zinc-dependent alcohol dehydrogenase -
  D9777_RS03040 - 577737..578171 (+) 435 WP_021494203.1 RDD family protein -
  D9777_RS03045 - 578243..578566 (+) 324 WP_007409357.1 YckD family protein -
  D9777_RS03050 - 578670..580106 (+) 1437 WP_021494204.1 family 1 glycosylhydrolase -
  D9777_RS03055 nin/comJ 580147..580545 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  D9777_RS03060 nucA/comI 580566..581003 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  D9777_RS03065 hxlB 581334..581891 (-) 558 WP_021494205.1 6-phospho-3-hexuloisomerase -
  D9777_RS03070 hxlA 581888..582523 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  D9777_RS03075 - 582755..583117 (+) 363 WP_021494206.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=320908 D9777_RS03060 WP_003156588.1 580566..581003(-) (nucA/comI) [Bacillus velezensis strain Bac57]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=320908 D9777_RS03060 WP_003156588.1 580566..581003(-) (nucA/comI) [Bacillus velezensis strain Bac57]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment