Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EST53_RS10405 Genome accession   NZ_CP040269
Coordinates   2142802..2143242 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain 1500     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2137802..2148242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EST53_RS10385 (EST53_10385) aroP 2138800..2140173 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EST53_RS10390 (EST53_10390) ampE 2140216..2141070 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EST53_RS10395 (EST53_10395) ampD 2141067..2141618 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EST53_RS10400 (EST53_10400) nadC 2141706..2142599 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EST53_RS10405 (EST53_10405) pilA 2142802..2143242 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  EST53_RS10410 (EST53_10410) pilB 2143252..2144637 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  EST53_RS10415 (EST53_10415) hofC 2144627..2145829 (+) 1203 WP_000157234.1 protein transport protein HofC -
  EST53_RS10420 (EST53_10420) guaC 2145864..2146907 (-) 1044 WP_001217338.1 GMP reductase -
  EST53_RS23570 - 2147063..2147107 (-) 45 WP_120795372.1 protein YacM -
  EST53_RS10425 (EST53_10425) coaE 2147132..2147752 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=320907 EST53_RS10405 WP_000360895.1 2142802..2143242(+) (pilA) [Escherichia coli strain 1500]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=320907 EST53_RS10405 WP_000360895.1 2142802..2143242(+) (pilA) [Escherichia coli strain 1500]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment