Detailed information NTG0320845

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: D9779_RS06680
• Genome accession: NZ_CP033052
• Coordinates: 1308604..1312308 (+)
• Length: 1234 a.a.
• NCBI ID: WP_121642273.1
• Uniprot ID: -
• Organism: Bacillus vallismortis strain Bac111
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1303604..1317308

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9779_RS06675	addB	1305117..1308617 (+)	3501	WP_121642272.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
D9779_RS06680	addA	1308604..1312308 (+)	3705	WP_121642273.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
D9779_RS06685	sbcD	1312382..1313557 (+)	1176	WP_121642274.1	exonuclease subunit SbcD	-
D9779_RS06690	sbcC	1313554..1316946 (+)	3393	WP_121642275.1	exonuclease subunit SbcC	-
D9779_RS06695	-	1316960..1317262 (+)	303	WP_121642276.1	HNH endonuclease	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141554.95 Da
• Isoelectric Point: 5.0963

>NTDB_id=320845 D9779_RS06680 WP_121642273.1 1308604..1312308(+) (addA) [Bacillus vallismortis strain Bac111]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVNRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSKKSAIEELPFYQYIKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDEFNELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLIRLVTDFGKRFEAAKQEKSIIDFSDLEHYCLSILTTEDDKGEQKPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RTDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEETDANEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMDELRAQGIPVYANLTSGYFDAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYTHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYIGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQVAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHKDVEDLAENVMAAPADISSHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRRGEPVPGSFTFDEKAREQLSWTYPHHEVTHIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTVEEAEQTVHKLFEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETENGLYLLDYKSDRIEGKFQHGFEGAVPILKKRYE
TQIQLYTKAVEQIANTNVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=320845 D9779_RS06680 WP_121642273.1 1308604..1312308(+) (addA) [Bacillus vallismortis strain Bac111]
ATGAACATTCCTAAACCGGCAGACAGCACTTGGACAGATGACCAATGGAATGCCATTGTTTCAACGGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGAAAAACCGCTGTGCTCGTCGAACGAATGATCCGGAAGATCACCGCGGAGGAAAACC
CGATTGATGTGGACCGGCTTCTCGTTGTGACGTTTACAAATGCCTCAGCGGCAGAGATGAAACACCGGATTGCGGATGCC
TTGGAAAAAGAGCTTGTCAACCGGCCAGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACAGGGCCAGCATTTC
GACACTGCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAAACAGAAGGCGAACTTTTGGGGGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAAGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCATGATCTGGACCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCAAATCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGAAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATATCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTCCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGTCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTCGCGCAGATTGATGAACT
GATTCAGCATCAGGATGAATTCAATGAGCTTTATAAGCGGGTGCCGGCAGTCTCCTTTAAGCGCGCGAAAGCGGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACGAGAAGTCCTGAACAGCATTTGAAAAGCTTAGCCGAGATGAAGCCTGTCATTGAAACGCTCATACG
GCTTGTCACCGACTTTGGAAAACGGTTCGAAGCCGCGAAACAGGAAAAATCAATCATTGATTTTTCTGATTTAGAGCATT
ACTGTTTATCCATTTTGACAACTGAGGATGATAAAGGTGAACAGAAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAAGATACCAACTTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
CGAGGAAACAGGCAACCTATTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGTTGGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAAAGCGGAGAAGGCACGGGCCGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGGACTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGATGA
GCAGGCTGAGCTGAAGCTTGGCGCGGCGTATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCCG
AAGAGACGGATGCAAACGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTTGAAGCGAAAGCGATCGCCAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAGTACCGCGATATCGTGAT
TTTGCTTCGTTCCATGCCGTGGGCGCCGCAAATCATGGACGAGCTGAGGGCGCAGGGTATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTTGATGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAATCCGTATCAGGATATA
CCGCTGGCCTCTGTGTTGCGCTCACCTATTGTCGGGGCGGATGAAAACGAGCTGTCTTTGATCAGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGATTACTTGGCGGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACAT
TCTACACTCACCTGCAAAAATGGCGCGCCTTTTCAAAAAACCACTCCGTATCCGAACTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATATCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCCCGCGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTATATTCATCCGCAATTGCGCATTAGCTATCCGACGCTGCCACTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTTGCCTTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCATGCAAGGATCATCAGAAACAGGTCGCAAAATGGCAGGCATCTGCCTCCCAAACGGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGTCCGGCTCTTGCCAGACACAAAGATGTAGAGGATTTGG
CTGAAAACGTTATGGCAGCGCCTGCCGACATATCAAGCCACCCGGCTCGTTTTGCCGTTCGTATGATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGTTTAGAAGCGATACGCCGTGGTGAACCAGTTCC
TGGCTCGTTTACGTTTGATGAAAAAGCCCGTGAGCAGCTGAGCTGGACATATCCTCATCACGAGGTGACGCACATTCGGA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCT
GACGGAAGCATGCTGTACAGGCGGCCCGCTTTCATGATGAAAAAAGGCCTGACAGCGGCTGAAAAAGGGACGGCCATGCA
TACAGTGATGCAGCATATTCCGCTGACACATGTGCCGACAGTAGAGGAAGCGGAGCAAACGGTTCATAAGCTCTTTGAAA
AGGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCGTTTAGTTTGGCGCTTCCCGCCAAAGAAATCTACCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTGGTGCAGGGAATTATCGACTGTCTCTATGAAACTGAAAACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGGGCGGTCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCGAACACAAACGTAAAGGGATGTGCGCTTTATTTCTTTGA
TGGAGGTCACATTCTGACATTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	95.219	100	0.952