Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   D9779_RS06675 Genome accession   NZ_CP033052
Coordinates   1305117..1308617 (+) Length   1166 a.a.
NCBI ID   WP_121642272.1    Uniprot ID   -
Organism   Bacillus vallismortis strain Bac111     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1300117..1313617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9779_RS06650 - 1300241..1300324 (+) 84 Protein_1236 MarR family transcriptional regulator -
  D9779_RS06655 - 1300350..1300520 (-) 171 WP_010329295.1 hypothetical protein -
  D9779_RS06660 - 1300573..1301727 (-) 1155 WP_121642269.1 AbrB family transcriptional regulator -
  D9779_RS06665 - 1301835..1303037 (-) 1203 WP_121642270.1 MDR family MFS transporter -
  D9779_RS06670 - 1303153..1304880 (+) 1728 WP_121642271.1 SgrR family transcriptional regulator -
  D9779_RS06675 addB 1305117..1308617 (+) 3501 WP_121642272.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D9779_RS06680 addA 1308604..1312308 (+) 3705 WP_121642273.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D9779_RS06685 sbcD 1312382..1313557 (+) 1176 WP_121642274.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134824.84 Da        Isoelectric Point: 5.5787

>NTDB_id=320844 D9779_RS06675 WP_121642272.1 1305117..1308617(+) (addB) [Bacillus vallismortis strain Bac111]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTVSEYRGERVLSE
KLHDLSILYQQMEKSLSEQYLHSEDYLTLLAEHIPLSEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDIAYKELSGTQRHERTPELAHLEAQYEARPAIPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDIAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVREKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKNIGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHMERLLTNEPEQVSDEEQLKYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSQPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCSFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=320844 D9779_RS06675 WP_121642272.1 1305117..1308617(+) (addB) [Bacillus vallismortis strain Bac111]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATACAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTATTCAGCTTTTCACGTTTGGCCTGGCGCGTCCTCCAGCATACAGGG
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGATTTACCGCACAGGTTGAACGGATGTTGACCGAGTTTAAGCGTTACT
GTCTGGAGCCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGTTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTTCATGACCTATCAATTTTGTACCAGCAGATGGAAAAAAGCCTTTCAGAGCAATATCTTCACTCCGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTGTCTGAAGATGTAAAAGGCGCGCATATCTACGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTGATGGTTCATGCAGAGCACATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATACGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATTGCTTATAAAGAGCTAAGCGGGACTCAGCGGCATGAGCGCACACCGGAACTGGCGCATTTGG
AGGCGCAGTATGAAGCGCGCCCGGCCATTCCGTACGCCGAAAAACAAGAGGCTTTTACGGTCATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCGCTTGTCAGAGAGAAGGGTTATCGCTATAAGGATATAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTAAAGGAAGTTTTCGCGGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCCATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGGTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAAACGAAGGTGAGAGAACAGGTTGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATTAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTTCGGGAGAAGGCGGAGGCACTCTACCG
CTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAAGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCCGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCCCTTGATTTGTTTCAGCAAATGATGGAAGCAGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTACGGCACCTCCTGCACCTTTGTGCTGGGGGCAAACGATGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTGCTGTCGGATGATGACCGGGAATGGCTGAAAAACATAGGGATTGAGCTTTCC
TCGGGTGGCCGGGAACGTCTCCTTGATGAGCATTTCCTCATTTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCATTAAGCGGCTGGAGGAACTGTTTCCTC
AGCACATGGAGCGCCTGTTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTTAAGTACGTCGTCAATAAAAGC
GTGGCACAGTCTTTTACCGCGAGCCAGCTCAGATTATGGACGCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
TAATGTGCTGATGAGTCAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGAAATGAAGTAAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGCGAACGCATTCAGGGCAGTGTGTCGAGAATGGAAACCTTTAACGCG
TGCCCATTCTCCCATTTTGCTTCACACGGGCTGCATCTCAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACAAAAG
AGCAATGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCCCCAAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCTGGAATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTTGTGCCGATCGGGCTAGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTACACTGAAAAACG
GCTGCACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAAGCAGAAAGCTCAAAAGGTTTGCTTCTAAGGATTGTC
GATTATAAATCTAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTATCTTGA
TTTATCAATTACACATTCAGCCGACTGGCTCGGCATGAGAGCGACGCCGGCCGGAGTGCTGTATTTTCATATCCATGACC
CGATGATTCAATCTAATCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATTTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCGGCAGTCGGCGAAAAGGAGTTTGACCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAGGCAGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTTCGTTCAAATCAGTATGCCAATTCGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.256

100

0.973

Multiple sequence alignment