Detailed information NTG0320489

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D9M09_RS02120
• Genome accession: NZ_CP033019
• Coordinates: 453914..455053 (-)
• Length: 379 a.a.
• NCBI ID: WP_205602324.1
• Uniprot ID: -
• Organism: Janthinobacterium agaricidamnosum strain BHSEK
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 448914..460053

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9M09_RS02105 (D9M09_02105)	glcF	449906..451141 (+)	1236	WP_121668470.1	glycolate oxidase subunit GlcF	-
D9M09_RS02110 (D9M09_02110)	-	451134..453242 (-)	2109	WP_121668471.1	EAL domain-containing protein	-
D9M09_RS02115 (D9M09_02115)	-	453427..453912 (-)	486	WP_121668472.1	glutathione peroxidase	-
D9M09_RS02120 (D9M09_02120)	pilU	453914..455053 (-)	1140	WP_205602324.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D9M09_RS02125 (D9M09_02125)	pilT	455057..456100 (-)	1044	WP_070312390.1	type IV pilus twitching motility protein PilT	Machinery gene
D9M09_RS02130 (D9M09_02130)	-	456171..456872 (+)	702	WP_070290071.1	YggS family pyridoxal phosphate-dependent enzyme	-
D9M09_RS02135 (D9M09_02135)	proC	456889..457719 (+)	831	WP_121668473.1	pyrroline-5-carboxylate reductase	-
D9M09_RS02140 (D9M09_02140)	-	457732..458448 (-)	717	WP_121668474.1	anti-sigma factor	-
D9M09_RS02145 (D9M09_02145)	-	458445..459011 (-)	567	WP_070290077.1	sigma-70 family RNA polymerase sigma factor	-
D9M09_RS02150 (D9M09_02150)	-	459102..459659 (-)	558	WP_070312386.1	DUF3455 domain-containing protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 40994.13 Da
• Isoelectric Point: 6.9413

>NTDB_id=320489 D9M09_RS02120 WP_205602324.1 453914..455053(-) (pilU) [Janthinobacterium agaricidamnosum strain BHSEK]
MQTTHEHYGAMHALLAQMRARGGSDLFITAGFPPAIKLDGKLTPLAGAALAAQQAAGYVRAVMNERQAAEFESSREANFA
ISPDGLGRFRVSAFVQMGQAGMVLRLINTAIPTLDGLGLPAILQDIVMSKRGLVIMVGATGCGKSTTLAAMVGHRNAHSH
GHIITIEDPVEFIHPHGNCIVTQREVGVDTDDWATALKNTLRQAPDVIQIGEIRDRDTMDHAIAFAETGHLCLATLHANN
ANQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPNKEGGGRKAALEILLNSPLMSDLIFKGQVHEIKELMKKSREH
GMQTFDQALFDLHEAGAISHEDALRNADSVNDLRLAIKLKGATAPEPAPQTGATKLGLL

Nucleotide

• Length: 1140 bp

>NTDB_id=320489 D9M09_RS02120 WP_205602324.1 453914..455053(-) (pilU) [Janthinobacterium agaricidamnosum strain BHSEK]
ATGCAGACGACGCACGAACACTATGGCGCCATGCACGCGCTGCTGGCGCAGATGCGCGCACGGGGCGGCTCCGACCTGTT
CATCACGGCCGGGTTTCCGCCCGCCATCAAGCTCGACGGCAAGCTGACGCCGCTGGCGGGCGCCGCGCTGGCCGCGCAGC
AGGCGGCCGGCTACGTGCGCGCCGTCATGAACGAACGCCAGGCGGCCGAGTTCGAATCGAGCCGCGAAGCCAATTTCGCC
ATCAGCCCGGACGGCCTCGGGCGTTTCCGCGTGTCCGCCTTCGTGCAGATGGGCCAGGCAGGCATGGTCTTGCGACTGAT
CAATACCGCCATCCCCACGCTCGATGGGCTCGGTTTACCCGCCATCCTGCAAGACATCGTGATGAGCAAGCGGGGCCTCG
TCATCATGGTGGGCGCCACGGGCTGCGGCAAGTCGACCACCCTGGCCGCCATGGTGGGCCACCGCAACGCGCACAGCCAT
GGCCACATCATCACCATCGAAGACCCCGTGGAATTCATCCATCCGCACGGCAACTGCATCGTCACCCAGCGCGAGGTGGG
CGTCGACACGGACGACTGGGCCACGGCCCTGAAAAACACCTTGCGCCAGGCGCCCGACGTGATCCAGATCGGCGAAATAC
GCGACCGCGACACGATGGACCACGCCATCGCATTTGCCGAGACGGGCCATCTGTGCCTGGCCACCCTGCACGCGAACAAC
GCCAACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAAGAGCGTCGCCAGCAACTGCTGATGGATCTGTCGCTGAA
TTTGAAGGGCATGATTTCGCAGCGTTTGATCCCCAACAAGGAAGGCGGCGGGCGCAAGGCGGCGCTGGAAATCCTGCTCA
ATTCGCCGCTGATGAGCGACCTGATCTTCAAGGGTCAGGTGCACGAGATCAAGGAATTGATGAAAAAATCGCGCGAGCAT
GGCATGCAGACGTTCGACCAGGCCCTGTTCGACCTGCACGAAGCGGGCGCCATCAGCCATGAAGACGCGCTGCGCAATGC
GGACTCCGTCAACGACCTGCGCCTGGCGATCAAACTCAAGGGCGCCACGGCGCCGGAACCCGCTCCGCAAACGGGCGCCA
CCAAACTGGGACTCCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.173	93.14	0.588
pilU	Acinetobacter baylyi ADP1	59.078	91.557	0.541
pilU	Vibrio cholerae strain A1552	56	92.348	0.517
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.425	86.28	0.375