Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   TMZ1T_RS11140 Genome accession   NC_011662
Coordinates   2537751..2539169 (-) Length   472 a.a.
NCBI ID   WP_004322149.1    Uniprot ID   N6XW56
Organism   Thauera aminoaromatica     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2532751..2544169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS11120 (Tmz1t_2248) - 2534198..2534929 (+) 732 WP_004302164.1 2Fe-2S iron-sulfur cluster-binding protein -
  TMZ1T_RS11125 (Tmz1t_2249) - 2534926..2535507 (+) 582 WP_012585447.1 NADP oxidoreductase -
  TMZ1T_RS11130 (Tmz1t_2250) - 2535616..2537082 (+) 1467 WP_012585448.1 Ni/Fe hydrogenase subunit alpha -
  TMZ1T_RS11135 (Tmz1t_2251) - 2537079..2537522 (+) 444 WP_004322151.1 hydrogenase maturation protease -
  TMZ1T_RS11140 (Tmz1t_2252) radA 2537751..2539169 (-) 1419 WP_004322149.1 DNA repair protein RadA Machinery gene
  TMZ1T_RS11145 (Tmz1t_2253) - 2539231..2540142 (-) 912 WP_012585449.1 LysR family transcriptional regulator -
  TMZ1T_RS11150 (Tmz1t_2254) lplT 2540360..2541610 (+) 1251 WP_004322141.1 lysophospholipid transporter LplT -
  TMZ1T_RS11155 (Tmz1t_2255) - 2541659..2542573 (-) 915 WP_004322138.1 uracil-DNA glycosylase -
  TMZ1T_RS11160 (Tmz1t_2256) rimI 2542573..2543019 (-) 447 WP_004322136.1 ribosomal protein S18-alanine N-acetyltransferase -
  TMZ1T_RS11165 (Tmz1t_2257) tsaB 2543019..2543711 (-) 693 WP_004322135.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 50162.50 Da        Isoelectric Point: 7.6696

>NTDB_id=32010 TMZ1T_RS11140 WP_004322149.1 2537751..2539169(-) (radA) [Thauera aminoaromatica]
MARQKSAFVCTECGAQAVRWQGQCPQCQAWNTLVETVLEPAAPAAGSRFAALAGETSRLQPLSALEPREEPRQPTGLDEF
DRVLGGGLVAGGVVLIGGDPGIGKSTLLLQALSNLAATSASAGSEGATGKGTKAGGVIYVSGEESGEQVALRAQRLQLPQ
SPLQMLAEINLERILHTLREARPRVAVIDSIQTVYSETLQSAPGSVAQVRECAAQLTRFAKQSGTSLILVGHVTKDGTLA
GPRVLEHIVDTVLYFEGDTHSSFRLIRAFKNRFGAVNELGVFAMTERGLRGVSNPSAIFLSQHSQQVAGSCVLITQEGTR
PLLVEVQALVDSSQSPNPRRLSVGLEQNRLAMLLAVMHRHAGVVCFDQDVFVNAVGGVKITEPAADLAILFAIVSSLRDR
PLRRGLAVFGEVGLAGEIRPAPRGQERLKEAAKLGFDTAIVPRANAPKHPIEGLKVIAVERVEEALEQLRGL

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=32010 TMZ1T_RS11140 WP_004322149.1 2537751..2539169(-) (radA) [Thauera aminoaromatica]
ATGGCACGGCAGAAATCCGCTTTCGTCTGCACCGAATGCGGTGCGCAGGCCGTGCGCTGGCAAGGCCAGTGCCCGCAGTG
CCAGGCCTGGAACACCCTTGTCGAAACCGTGCTCGAGCCCGCAGCGCCGGCTGCGGGCAGCCGCTTCGCCGCGCTCGCCG
GCGAGACCAGCCGGCTGCAGCCCCTGTCCGCGCTCGAACCCCGCGAAGAACCCCGCCAGCCCACCGGTCTCGACGAGTTC
GACCGCGTGCTCGGTGGCGGCCTGGTCGCCGGCGGCGTGGTGCTGATCGGCGGCGACCCGGGTATCGGAAAATCCACCCT
GCTGCTGCAGGCGCTCTCCAACCTGGCCGCGACGAGTGCGTCGGCTGGCAGCGAGGGCGCGACGGGCAAGGGCACCAAGG
CGGGCGGCGTCATCTACGTCAGCGGCGAGGAGTCGGGCGAGCAGGTCGCGCTGCGTGCCCAGCGCCTGCAGTTACCGCAG
TCGCCCCTGCAGATGCTCGCCGAGATCAACCTCGAACGCATCCTCCACACCCTGCGCGAGGCGCGCCCGCGCGTGGCGGT
CATCGACTCGATCCAGACCGTCTATTCCGAGACCCTGCAGTCCGCCCCCGGCTCGGTCGCCCAAGTGCGCGAATGCGCCG
CCCAGCTCACCCGCTTTGCCAAGCAGAGCGGAACCAGCCTGATCCTGGTCGGCCACGTCACCAAGGACGGCACGCTCGCC
GGCCCGCGCGTGCTCGAGCACATCGTAGACACCGTGCTGTACTTCGAGGGCGACACGCACTCCAGCTTCCGCCTGATCCG
CGCCTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTTCGCCATGACCGAGCGCGGCCTGCGCGGTGTGTCCA
ATCCCTCCGCGATCTTCCTGTCGCAGCACTCGCAGCAGGTCGCCGGCAGCTGCGTGCTGATCACCCAGGAGGGCACGCGC
CCGCTGCTGGTCGAGGTGCAGGCCTTGGTCGATTCGTCGCAGAGCCCCAACCCGCGCCGGCTGTCGGTGGGCCTGGAGCA
GAACCGCCTGGCGATGCTGCTCGCGGTCATGCACCGTCACGCCGGCGTGGTCTGCTTCGACCAGGACGTTTTCGTGAATG
CGGTCGGCGGCGTCAAGATCACCGAGCCGGCCGCCGACCTCGCGATCCTGTTCGCGATCGTCTCCTCGCTGCGCGACCGC
CCGCTTCGGCGCGGCCTCGCGGTGTTCGGCGAGGTCGGTCTCGCCGGCGAGATCCGGCCCGCACCGCGTGGCCAGGAGCG
CCTGAAGGAAGCCGCCAAGCTCGGCTTCGACACCGCCATCGTTCCGCGCGCCAATGCGCCGAAGCATCCGATCGAGGGCC
TGAAGGTGATCGCGGTCGAGCGCGTGGAGGAGGCCCTGGAGCAGTTGCGCGGGTTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-237)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(376-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6XW56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.408

99.788

0.483
  radA Streptococcus mitis SK321

43.737

99.788

0.436
  radA Streptococcus pneumoniae Rx1

43.46

100

0.436
  radA Streptococcus pneumoniae TIGR4

43.46

100

0.436
  radA Streptococcus pneumoniae D39

43.46

100

0.436
  radA Streptococcus pneumoniae R6

43.46

100

0.436
  radA Streptococcus mitis NCTC 12261

43.617

99.576

0.434

Multiple sequence alignment