Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   TMZ1T_RS03445 Genome accession   NC_011662
Coordinates   791368..792822 (-) Length   484 a.a.
NCBI ID   WP_004306755.1    Uniprot ID   N6YU78
Organism   Thauera aminoaromatica     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786368..797822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS03420 (Tmz1t_0703) - 787264..787854 (+) 591 WP_012584593.1 5-formyltetrahydrofolate cyclo-ligase -
  TMZ1T_RS03425 (Tmz1t_0704) ilvA 787892..789415 (-) 1524 WP_004306745.1 threonine ammonia-lyase, biosynthetic -
  TMZ1T_RS03430 (Tmz1t_0705) hprK 789488..790435 (-) 948 WP_004306747.1 HPr(Ser) kinase/phosphatase -
  TMZ1T_RS03435 (Tmz1t_0706) ptsN 790404..790889 (-) 486 WP_012584594.1 PTS IIA-like nitrogen regulatory protein PtsN -
  TMZ1T_RS03440 (Tmz1t_0707) hpf 791026..791349 (-) 324 WP_004306751.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  TMZ1T_RS03445 (Tmz1t_0708) rpoN/rpoN1 791368..792822 (-) 1455 WP_004306755.1 RNA polymerase factor sigma-54 Machinery gene
  TMZ1T_RS03450 (Tmz1t_0709) lptB 792845..793567 (-) 723 WP_004306760.1 LPS export ABC transporter ATP-binding protein -
  TMZ1T_RS03455 (Tmz1t_0710) recX 793554..794003 (-) 450 WP_004306763.1 recombination regulator RecX -
  TMZ1T_RS03460 (Tmz1t_0711) recA 794020..795057 (-) 1038 WP_004306766.1 recombinase RecA Machinery gene
  TMZ1T_RS03465 (Tmz1t_0712) pncC 795254..795742 (-) 489 WP_012584595.1 nicotinamide-nucleotide amidase -
  TMZ1T_RS03470 (Tmz1t_0713) - 795747..796229 (-) 483 WP_004306772.1 phosphatidylglycerophosphatase A -
  TMZ1T_RS03475 (Tmz1t_0714) thiL 796261..797220 (-) 960 WP_012584596.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 53494.32 Da        Isoelectric Point: 4.6476

>NTDB_id=32004 TMZ1T_RS03445 WP_004306755.1 791368..792822(-) (rpoN/rpoN1) [Thauera aminoaromatica]
MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTIELNQEIERVLLENPMLEREDGDGGVDYAPAPPPGNAPEREAAPEPAAEP
AGESITHEGGEGSSEDEGIDWSNVGQGASSRSEDDEDGDYQDIQAAGVSLREHLDQQVALSPLSDRDRALVRFLIEALDD
DGYLHQPLEDLLELLPQDAEVDLDELAIALRHVQSLEPAGIGARSPQECLALQLQALPECPIRPLALEIVTSHLELLAER
NFARIRKLTGCDDEGLREAQALICSLDPHPGSRYSTAETRYVLPDVVVRKLRGQWTVSLNQEAMPRLRINRLYASLLQQN
RAQGGGLGGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKYMAT
PRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDSKKPLSDAKIAEILGQQGIVVARRTIAKYRESLNIPPVSL
RKSL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=32004 TMZ1T_RS03445 WP_004306755.1 791368..792822(-) (rpoN/rpoN1) [Thauera aminoaromatica]
ATGAAACCCAGCCTCCAGCTCAAGCTCTCCCAGCACCTCACGCTGACGCCCCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCTACGATCGAGCTGAACCAGGAGATCGAGCGCGTGCTGCTCGAGAATCCCATGCTCGAGCGCGAGGATGGCGACG
GCGGCGTCGACTACGCCCCCGCGCCGCCGCCGGGCAACGCGCCCGAGCGCGAAGCCGCGCCGGAACCCGCCGCCGAACCG
GCGGGCGAGTCGATCACGCACGAGGGCGGCGAAGGCAGCAGCGAGGACGAGGGCATCGACTGGTCCAACGTGGGTCAGGG
CGCGTCCAGCCGCAGCGAGGACGACGAGGACGGCGACTACCAGGACATCCAGGCCGCCGGCGTGTCGCTGCGCGAACACC
TCGACCAGCAGGTCGCCCTCTCGCCCCTGTCCGACCGCGACCGCGCGCTGGTGCGCTTCCTGATCGAGGCACTCGACGAC
GACGGCTACCTGCACCAGCCGCTGGAAGATCTCCTCGAGCTGTTGCCGCAGGACGCCGAGGTCGATCTCGACGAGCTCGC
CATCGCCCTGCGCCACGTGCAGAGCCTCGAGCCGGCCGGGATCGGCGCGCGCAGCCCGCAGGAATGTCTGGCCCTGCAGC
TCCAGGCCTTGCCCGAGTGCCCGATACGCCCTCTCGCTCTCGAGATCGTGACCAGCCACCTCGAACTGCTCGCGGAGCGC
AACTTCGCCCGTATCCGCAAGCTCACCGGCTGTGACGACGAGGGTCTGCGCGAAGCGCAGGCCCTGATCTGCAGCCTCGA
CCCCCACCCCGGCTCGCGCTACTCCACCGCCGAGACGCGCTACGTGCTGCCCGACGTCGTCGTGCGCAAGCTGCGCGGCC
AATGGACCGTGAGCCTGAACCAGGAAGCGATGCCCCGCCTGCGCATCAACCGCCTGTACGCGAGCCTGTTGCAGCAGAAC
CGCGCACAGGGCGGCGGCCTCGGCGGGCAGCTGCAGGAGGCGCGCTGGCTGATCAAGAACGTCCAGCAGCGCTTCGACAC
CATCCTGCGCGTGTCCCAGGCCATCGTTGACCAGCAACGGCAGTTCTTCGATCATGGGGACGTGGCGATGCGGCCACTCA
CCCTAAGGGAGATCGCAGACCAGCTCGAGCTGCACGAATCCACCGTCTCGCGCGTCACCACGCAGAAGTACATGGCGACG
CCGCGCGGAGTCTTCGAGCTCAAGTATTTTTTCGGCAGTCATGTCGCGACCGACACCGGCGGTGCGGCTTCCTCCACGGC
GATTCGTGCGCTGATTCGCCAGCTGGTGGACGCGGAGGACAGCAAGAAGCCGCTGTCCGACGCCAAGATCGCCGAAATTC
TCGGCCAACAGGGAATCGTCGTGGCGAGGCGCACGATCGCCAAGTACCGCGAGTCCCTCAACATCCCGCCGGTCAGCCTG
CGCAAATCCCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(330-482)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-313)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6YU78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.455

100

0.597

Multiple sequence alignment