Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   D9C20_RS18810 Genome accession   NZ_CP032867
Coordinates   3537256..3538149 (+) Length   297 a.a.
NCBI ID   WP_014479775.1    Uniprot ID   A0AA96ZWR3
Organism   Bacillus subtilis subsp. subtilis strain N4-2     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3532256..3543149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C20_RS18790 (D9C20_18790) - 3532926..3534656 (+) 1731 WP_014479774.1 hypothetical protein -
  D9C20_RS18795 (D9C20_18795) ylqH 3534653..3534934 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  D9C20_RS18800 (D9C20_18800) sucC 3535107..3536264 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  D9C20_RS18805 (D9C20_18805) sucD 3536293..3537195 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  D9C20_RS18810 (D9C20_18810) dprA 3537256..3538149 (+) 894 WP_014479775.1 DNA-processing protein DprA Machinery gene
  D9C20_RS18815 (D9C20_18815) topA 3538337..3540412 (+) 2076 WP_014479776.1 type I DNA topoisomerase -
  D9C20_RS18820 (D9C20_18820) trmFO 3540488..3541795 (+) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  D9C20_RS18825 (D9C20_18825) xerC 3541863..3542777 (+) 915 WP_014479777.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32907.21 Da        Isoelectric Point: 9.9142

>NTDB_id=319839 D9C20_RS18810 WP_014479775.1 3537256..3538149(+) (dprA) [Bacillus subtilis subsp. subtilis strain N4-2]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=319839 D9C20_RS18810 WP_014479775.1 3537256..3538149(+) (dprA) [Bacillus subtilis subsp. subtilis strain N4-2]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACGAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

99.663

100

0.997
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment