Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   D9C18_RS12190 Genome accession   NZ_CP032865
Coordinates   2290401..2291546 (-) Length   381 a.a.
NCBI ID   WP_014481226.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain N3-1     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 2285401..2296546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C18_RS12165 (D9C18_12165) pbpG 2285668..2287743 (-) 2076 WP_014481227.1 transglycosylase domain-containing protein -
  D9C18_RS12170 (D9C18_12170) speE 2287945..2288775 (+) 831 WP_003227543.1 spermidine synthase -
  D9C18_RS12175 (D9C18_12175) speB 2288836..2289708 (+) 873 WP_003227545.1 agmatinase -
  D9C18_RS12180 (D9C18_12180) - 2289773..2290200 (-) 428 Protein_2332 YbaK/EbsC family protein -
  D9C18_RS12185 (D9C18_12185) phrF 2290298..2290417 (-) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  D9C18_RS12190 (D9C18_12190) rapF 2290401..2291546 (-) 1146 WP_014481226.1 response regulator aspartate phosphatase RapF Regulator
  D9C18_RS23280 (D9C18_12195) - 2291549..2291779 (+) 231 Protein_2335 hypothetical protein -
  D9C18_RS12200 (D9C18_12200) ywhK 2291776..2293131 (+) 1356 WP_014481224.1 YncE family protein -
  D9C18_RS12205 (D9C18_12205) ywhL 2293170..2294546 (+) 1377 WP_014481223.1 YncE family protein -
  D9C18_RS12210 (D9C18_12210) albG 2294552..2295253 (-) 702 WP_014481222.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  D9C18_RS12215 (D9C18_12215) albF 2295250..2296530 (-) 1281 WP_014481221.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45627.12 Da        Isoelectric Point: 5.1503

>NTDB_id=319726 D9C18_RS12190 WP_014481226.1 2290401..2291546(-) (rapF) [Bacillus subtilis subsp. subtilis strain N3-1]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=319726 D9C18_RS12190 WP_014481226.1 2290401..2291546(-) (rapF) [Bacillus subtilis subsp. subtilis strain N3-1]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_12)

(223-290)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

99.738

100

0.997
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572

Multiple sequence alignment