Detailed information NTG0319144

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: D9C09_RS01545
• Genome accession: NZ_CP032852
• Coordinates: 292437..293582 (+)
• Length: 381 a.a.
• NCBI ID: WP_069837832.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain GFR-12
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 287437..298582

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9C09_RS01520 (D9C09_01520)	albF	287457..288737 (+)	1281	WP_069837829.1	pitrilysin family protein	-
D9C09_RS01525 (D9C09_01525)	albG	288734..289435 (+)	702	WP_069837830.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
D9C09_RS01530 (D9C09_01530)	ywhL	289441..290817 (-)	1377	WP_069837831.1	YncE family protein	-
D9C09_RS01535 (D9C09_01535)	ywhK	290856..292207 (-)	1352	Protein_301	YncE family protein	-
D9C09_RS23200 (D9C09_01540)	-	292204..292434 (-)	231	Protein_302	hypothetical protein	-
D9C09_RS01545 (D9C09_01545)	rapF	292437..293582 (+)	1146	WP_069837832.1	response regulator aspartate phosphatase RapF	Regulator
D9C09_RS01550 (D9C09_01550)	phrF	293566..293685 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
D9C09_RS01555 (D9C09_01555)	-	293783..294210 (+)	428	Protein_305	YbaK/EbsC family protein	-
D9C09_RS01560 (D9C09_01560)	speB	294275..295147 (-)	873	WP_003227545.1	agmatinase	-
D9C09_RS01565 (D9C09_01565)	speE	295208..296038 (-)	831	WP_003227543.1	spermidine synthase	-
D9C09_RS01570 (D9C09_01570)	pbpG	296240..298315 (+)	2076	WP_069837833.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45624.07 Da
• Isoelectric Point: 5.0624

>NTDB_id=319144 D9C09_RS01545 WP_069837832.1 292437..293582(+) (rapF) [Bacillus subtilis subsp. subtilis strain GFR-12]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFTIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=319144 D9C09_RS01545 WP_069837832.1 292437..293582(+) (rapF) [Bacillus subtilis subsp. subtilis strain GFR-12]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACAGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCGTCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACATATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCACTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.688	100	0.987
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57