Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   D5S13_RS12335 Genome accession   NZ_CP032821
Coordinates   2466133..2466630 (+) Length   165 a.a.
NCBI ID   WP_029642894.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JDFM SA01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2461133..2471630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S13_RS12295 (D5S13_02346) - 2461177..2462163 (+) 987 WP_000161314.1 ROK family glucokinase -
  D5S13_RS12300 (D5S13_02347) - 2462163..2462492 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  D5S13_RS12305 (D5S13_02348) - 2462489..2463112 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  D5S13_RS12310 (D5S13_02349) comGA 2463164..2464138 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  D5S13_RS12315 (D5S13_02350) comGB 2464110..2465180 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  D5S13_RS12320 (D5S13_02351) comGC 2465194..2465505 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  D5S13_RS12325 (D5S13_02352) comGD 2465483..2465929 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  D5S13_RS12330 (D5S13_02353) comGE 2465916..2466215 (+) 300 WP_263258484.1 competence protein ComGE Machinery gene
  D5S13_RS12335 (D5S13_02354) comGF 2466133..2466630 (+) 498 WP_029642894.1 competence type IV pilus minor pilin ComGF Machinery gene
  D5S13_RS12340 - 2466727..2466873 (+) 147 WP_001789879.1 hypothetical protein -
  D5S13_RS12345 (D5S13_02355) - 2466863..2467387 (+) 525 WP_001015119.1 shikimate kinase -
  D5S13_RS12350 (D5S13_02356) gcvT 2467546..2468637 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  D5S13_RS12355 (D5S13_02357) gcvPA 2468657..2470003 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  D5S13_RS12360 (D5S13_02358) gcvPB 2469996..2471468 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19196.92 Da        Isoelectric Point: 10.2192

>NTDB_id=318998 D5S13_RS12335 WP_029642894.1 2466133..2466630(+) (comGF) [Staphylococcus aureus strain JDFM SA01]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=318998 D5S13_RS12335 WP_029642894.1 2466133..2466630(+) (comGF) [Staphylococcus aureus strain JDFM SA01]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGGCATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(70-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment