Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   D8S77_RS02670 Genome accession   NZ_CP032700
Coordinates   514881..516206 (+) Length   441 a.a.
NCBI ID   WP_032465157.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY556     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509881..521206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8S77_RS02645 (D8S77_02650) - 510651..511292 (+) 642 WP_002983671.1 response regulator transcription factor -
  D8S77_RS02650 (D8S77_02655) - 511329..512729 (+) 1401 WP_115354026.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  D8S77_RS02655 (D8S77_02660) - 512729..513106 (+) 378 WP_002983674.1 S1 RNA-binding domain-containing protein -
  D8S77_RS02660 (D8S77_02665) cysK 513124..514065 (-) 942 WP_009880467.1 cysteine synthase A -
  D8S77_RS02665 (D8S77_02670) - 514193..514825 (-) 633 WP_032465870.1 YigZ family protein -
  D8S77_RS02670 (D8S77_02675) comFA/cflA 514881..516206 (+) 1326 WP_032465157.1 DEAD/DEAH box helicase Machinery gene
  D8S77_RS02675 (D8S77_02680) - 516178..516843 (+) 666 WP_063632027.1 ComF family protein -
  D8S77_RS02680 (D8S77_02685) hpf 516923..517471 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49788.34 Da        Isoelectric Point: 10.0009

>NTDB_id=318318 D8S77_RS02670 WP_032465157.1 514881..516206(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY556]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWGGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=318318 D8S77_RS02670 WP_032465157.1 514881..516206(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY556]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGGGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCAAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-244)

Helicase conserved C-terminal domain (Helicase_C)

(289-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.727

89.796

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.31

98.639

0.388

Multiple sequence alignment