Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   CFK76_RS03455 Genome accession   NZ_CP032652
Coordinates   672750..674816 (+) Length   688 a.a.
NCBI ID   WP_226474266.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain LZ217     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 667750..679816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK76_RS03425 (CFK76_03425) - 668818..669108 (+) 291 WP_011374775.1 YlbG family protein -
  CFK76_RS03430 (CFK76_03430) rsmD 669113..669667 (+) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CFK76_RS03435 (CFK76_03435) coaD 669680..670177 (+) 498 WP_061826982.1 pantetheine-phosphate adenylyltransferase -
  CFK76_RS03440 (CFK76_03440) - 670179..671225 (+) 1047 WP_061826983.1 SepM family pheromone-processing serine protease -
  CFK76_RS03445 (CFK76_03445) comEA 671299..672006 (+) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  CFK76_RS03450 (CFK76_03450) - 672064..672552 (+) 489 WP_011374770.1 ComE operon protein 2 -
  CFK76_RS03455 (CFK76_03455) comEC 672750..674816 (+) 2067 WP_226474266.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CFK76_RS03460 (CFK76_03460) holA 674813..675841 (+) 1029 WP_061826985.1 DNA polymerase III subunit delta -
  CFK76_RS03465 (CFK76_03465) rpsT 675910..676164 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  CFK76_RS03470 (CFK76_03470) rpsO 676443..676712 (+) 270 WP_011374766.1 30S ribosomal protein S15 -
  CFK76_RS03475 (CFK76_03475) - 676940..678658 (+) 1719 WP_011374765.1 ribonuclease J -
  CFK76_RS03480 (CFK76_03480) - 678747..679694 (+) 948 WP_025015736.1 hypothetical protein -

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 77620.70 Da        Isoelectric Point: 9.7835

>NTDB_id=317592 CFK76_RS03455 WP_226474266.1 672750..674816(+) (comEC) [Latilactobacillus sakei strain LZ217]
MTQPQQPVAQYFKVQPDAIQVAGDSVQLTAIGQQDGQTIKAYYRCQTFAEKRQWQQVRHPVLFYGAKDFERIQGATNQNE
FDYARFLAQQKRCFYQLTLAKETTFALRRPSSGLDWLHYWRQTCALYLRKLPTALRFHAQTLLLGLREAQVDHYQVVLGR
LGIIHLLSLSGLHVFYLVQVIRWCATYCRIPREWLNCMLFGLLPVYALLVGGTTSISRAIALILCRLLCEQLGIRQSRLD
SWSLVLLVNLFWQPYLLHSMGGILSYLMAFALIYIGEGSTFKVAYWLSLLSLPVCLRFNYRWHVLTIMMNALVTPIYLPV
VLGLVIVASVMLPVSNLVVNGCEWLLQLIYKGLGLVAQIPHAMITFGKIPLLPLLLIVTVSLLLIDGQAISNQWRKRLKR
TLASLYLASFLAIHFNPTGQVVMFDIGQGDSLLIQTPFNRHQLLIDTGGRLALPQAAWQRRAQVSRAEKVTVNYLYSQGV
DHIDAVALSHQDADHIGDLNQILKQIRVDRIICAAGLPQNRQFQRQIRPFLATVQIEPYLAGTAFKVGPQKFNVLAPTKP
GKGENSDSLVLQAQIGGASWLFTGDLEQEGERAIIERYPQLTVDYLKVGHHGSKTASDPTVIKKLHLKGALISAGRENRY
GHPHQETLTTLQAAKVPYWLTAQQGMLIWAYGPGQSEKLQTTIKDSEK

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=317592 CFK76_RS03455 WP_226474266.1 672750..674816(+) (comEC) [Latilactobacillus sakei strain LZ217]
ATGACACAACCACAACAACCGGTAGCACAGTATTTTAAAGTGCAACCCGATGCGATTCAAGTCGCGGGCGACAGTGTTCA
ATTAACGGCAATCGGCCAACAAGATGGTCAGACTATTAAGGCTTATTATCGCTGCCAAACGTTCGCCGAGAAGAGGCAGT
GGCAACAAGTACGACACCCCGTTTTGTTCTATGGCGCAAAAGATTTTGAACGTATACAAGGAGCTACCAATCAAAATGAG
TTTGATTATGCCCGTTTTTTAGCGCAACAGAAGCGCTGCTTTTACCAACTGACGTTAGCCAAAGAAACAACTTTTGCGCT
GAGACGTCCTAGTAGCGGTTTGGATTGGCTACATTATTGGCGCCAAACGTGTGCGCTGTATTTACGTAAGTTACCAACAG
CACTACGGTTTCATGCGCAGACGTTACTATTAGGTTTGCGGGAAGCGCAAGTTGACCATTACCAGGTTGTTTTGGGCCGC
TTAGGGATTATTCATCTATTAAGTCTATCAGGGTTGCACGTGTTTTATTTAGTGCAAGTCATTCGGTGGTGTGCGACTTA
TTGCCGTATACCGCGTGAGTGGTTAAACTGTATGTTATTTGGGCTATTGCCGGTATATGCCTTACTGGTTGGTGGTACGA
CAAGCATTAGTCGCGCGATTGCGCTTATTTTATGCCGGTTACTTTGCGAACAGTTGGGCATTCGGCAATCAAGATTAGAT
AGTTGGAGTTTAGTATTACTGGTCAATTTATTCTGGCAGCCTTATTTGCTACATAGTATGGGCGGGATTTTAAGTTATTT
GATGGCCTTTGCGCTCATTTATATTGGTGAGGGTAGCACGTTTAAAGTTGCTTACTGGCTAAGTTTATTGAGTTTGCCGG
TTTGCTTACGCTTTAATTATCGCTGGCACGTGCTGACGATAATGATGAATGCCTTAGTAACGCCTATCTATCTACCAGTT
GTGCTAGGTTTGGTGATTGTAGCGAGTGTGATGTTACCTGTTAGCAATCTAGTAGTTAACGGATGTGAGTGGCTGTTACA
GTTGATTTACAAAGGCTTAGGATTAGTCGCTCAAATCCCCCATGCGATGATTACGTTTGGTAAAATCCCCTTGTTACCAT
TACTATTGATTGTGACGGTCAGCCTCTTGTTAATCGATGGCCAGGCCATTAGCAATCAGTGGCGTAAACGGCTGAAACGC
ACATTGGCAAGTTTGTATTTGGCTAGTTTTTTGGCGATTCATTTTAATCCGACTGGCCAGGTAGTCATGTTTGATATTGG
TCAGGGTGATAGTTTGTTGATTCAAACGCCGTTTAACCGGCATCAGCTTTTAATCGATACTGGTGGCCGCCTAGCGCTAC
CGCAAGCAGCTTGGCAACGGCGGGCGCAGGTTTCACGAGCGGAAAAAGTAACGGTTAATTATCTCTATAGCCAAGGAGTA
GATCACATTGATGCGGTGGCACTTTCTCATCAGGATGCCGATCATATTGGTGATTTGAATCAGATTTTGAAACAAATTCG
TGTTGATCGAATCATTTGTGCTGCCGGGTTACCGCAAAATCGGCAATTTCAAAGACAAATTCGACCATTTTTAGCAACGG
TTCAAATTGAACCCTATTTAGCCGGGACCGCTTTTAAAGTGGGGCCGCAAAAGTTTAACGTCTTAGCGCCGACTAAGCCG
GGAAAAGGCGAAAATAGTGATTCGTTAGTCTTACAGGCACAAATTGGTGGGGCTAGCTGGCTGTTTACAGGGGATTTAGA
GCAAGAAGGCGAACGAGCGATTATCGAGCGGTATCCACAGTTAACCGTCGATTATTTAAAAGTTGGCCATCACGGTAGTA
AAACGGCTAGTGACCCCACCGTGATTAAAAAATTGCACTTAAAAGGCGCATTAATCTCAGCCGGCAGAGAGAATCGTTAT
GGTCATCCGCACCAGGAAACTTTGACGACTTTGCAAGCGGCCAAAGTGCCTTATTGGTTAACGGCGCAGCAGGGGATGTT
GATCTGGGCGTACGGTCCCGGTCAAAGCGAAAAACTGCAAACAACAATAAAGGATAGTGAAAAATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(142-385)

Metallo-beta-lactamase superfamily (Lactamase_B)

(427-635)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Latilactobacillus sakei subsp. sakei 23K

97.384

100

0.974

Multiple sequence alignment