Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   CFK76_RS03445 Genome accession   NZ_CP032652
Coordinates   671299..672006 (+) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain LZ217     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666299..677006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK76_RS03415 (CFK76_03415) - 666508..667695 (+) 1188 WP_011374777.1 FtsW/RodA/SpoVE family cell cycle protein -
  CFK76_RS03420 (CFK76_03420) - 667706..668794 (+) 1089 WP_061826981.1 CAP-associated domain-containing protein -
  CFK76_RS03425 (CFK76_03425) - 668818..669108 (+) 291 WP_011374775.1 YlbG family protein -
  CFK76_RS03430 (CFK76_03430) rsmD 669113..669667 (+) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  CFK76_RS03435 (CFK76_03435) coaD 669680..670177 (+) 498 WP_061826982.1 pantetheine-phosphate adenylyltransferase -
  CFK76_RS03440 (CFK76_03440) - 670179..671225 (+) 1047 WP_061826983.1 SepM family pheromone-processing serine protease -
  CFK76_RS03445 (CFK76_03445) comEA 671299..672006 (+) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  CFK76_RS03450 (CFK76_03450) - 672064..672552 (+) 489 WP_011374770.1 ComE operon protein 2 -
  CFK76_RS03455 (CFK76_03455) comEC 672750..674816 (+) 2067 WP_226474266.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CFK76_RS03460 (CFK76_03460) holA 674813..675841 (+) 1029 WP_061826985.1 DNA polymerase III subunit delta -
  CFK76_RS03465 (CFK76_03465) rpsT 675910..676164 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  CFK76_RS03470 (CFK76_03470) rpsO 676443..676712 (+) 270 WP_011374766.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=317590 CFK76_RS03445 WP_226474265.1 671299..672006(+) (comEA) [Latilactobacillus sakei strain LZ217]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=317590 CFK76_RS03445 WP_226474265.1 671299..672006(+) (comEA) [Latilactobacillus sakei strain LZ217]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment