Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E7Z57_RS12980 Genome accession   NZ_CP039339
Coordinates   2714634..2716190 (+) Length   518 a.a.
NCBI ID   WP_064048275.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UW386     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2709634..2721190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7Z57_RS12965 (E7Z57_12965) gspM 2710712..2711290 (+) 579 WP_138929261.1 type II secretion system protein GspM -
  E7Z57_RS12970 (E7Z57_12970) - 2711294..2712145 (+) 852 WP_138929262.1 type II secretion system protein N -
  E7Z57_RS12975 (E7Z57_12975) gspD 2712179..2714599 (+) 2421 WP_064048272.1 type II secretion system secretin GspD -
  E7Z57_RS12980 (E7Z57_12980) pilF 2714634..2716190 (+) 1557 WP_064048275.1 type II secretion system ATPase GspE Machinery gene
  E7Z57_RS12985 (E7Z57_12985) gspF 2716192..2717403 (+) 1212 WP_064048271.1 type II secretion system inner membrane protein GspF -
  E7Z57_RS12990 (E7Z57_12990) - 2717794..2719077 (+) 1284 WP_138929263.1 hypothetical protein -
  E7Z57_RS12995 (E7Z57_12995) - 2719342..2720811 (-) 1470 WP_138929264.1 PLP-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55872.84 Da        Isoelectric Point: 5.4878

>NTDB_id=317344 E7Z57_RS12980 WP_064048275.1 2714634..2716190(+) (pilF) [Ralstonia pseudosolanacearum strain UW386]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDIDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLARFDHLINQPHGIVLVTGPTGSGKTTTLYAALSRLDAGSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CTHCRREEVLELTPTEIEAVAGTPPADGTAATVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=317344 E7Z57_RS12980 WP_064048275.1 2714634..2716190(+) (pilF) [Ralstonia pseudosolanacearum strain UW386]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATATCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAACTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTGGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCGCGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGTTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAGCGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGGCCGGTGGACGTGC
GCGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGTCTGCTCGACAAGGAAGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACACTGGCGCGCTTCGACCACCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCGGCGCTGTCGCGGCTCGATGCCGGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGCATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGACGTGGTGATGATCGGCGAAATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCACGCACTGCCGCCGCGAGGAAGTGCTGGAACTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCACCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAGCACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATTCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTGGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

43.056

97.297

0.419
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.883

99.228

0.396
  pilB Vibrio cholerae strain A1552

45.233

87.066

0.394
  pilB Vibrio campbellii strain DS40M4

44.42

88.224

0.392
  pilB/pilB1 Synechocystis sp. PCC 6803

44.907

83.398

0.375
  pilB Vibrio parahaemolyticus RIMD 2210633

46.078

78.764

0.363

Multiple sequence alignment