Detailed information NTG0317013

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: D7J84_RS13130
• Genome accession: NZ_CP032608
• Coordinates: 2577657..2579006 (+)
• Length: 449 a.a.
• NCBI ID: WP_061884753.1
• Uniprot ID: A0A9W7UZ89
• Organism: Bacillus thuringiensis strain QZL38
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2572657..2584006

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D7J84_RS13110 (D7J84_13140)	-	2573780..2574415 (-)	636	WP_061884755.1	YigZ family protein	-
D7J84_RS13115 (D7J84_13145)	-	2574652..2575494 (+)	843	WP_000684725.1	DegV family protein	-
D7J84_RS13120 (D7J84_13150)	-	2575667..2575981 (+)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
D7J84_RS13125 (D7J84_13155)	-	2576130..2577530 (+)	1401	WP_061884754.1	C40 family peptidase	-
D7J84_RS13130 (D7J84_13160)	comFA	2577657..2579006 (+)	1350	WP_061884753.1	ATP-dependent helicase ComFA	Machinery gene
D7J84_RS13135 (D7J84_13165)	-	2579006..2579710 (+)	705	WP_089148673.1	ComF family protein	-
D7J84_RS13140 (D7J84_13170)	cspC	2579837..2580034 (+)	198	WP_003303412.1	cold shock protein CspC	-
D7J84_RS13145 (D7J84_13175)	raiA	2580357..2580899 (+)	543	WP_000671186.1	ribosome-associated translation inhibitor RaiA	-
D7J84_RS13150 (D7J84_13180)	secA	2581172..2583679 (+)	2508	WP_061884751.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51193.04 Da
• Isoelectric Point: 9.7738

>NTDB_id=317013 D7J84_RS13130 WP_061884753.1 2577657..2579006(+) (comFA) [Bacillus thuringiensis strain QZL38]
MLAGKQLLLEELSSDLRKELSDLKKKGEVACVQGITKKASKYICQRCGNIEQRLFASFLCKRCSKRCTYCRKCITMGRVS
ECTVLVRGIQERKEERELNQLQWKGVLSTGQELVAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCVDQMLQYAVKQAM
KERAARIYLTATPDETWKRKLRKGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKIAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAEEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=317013 D7J84_RS13130 WP_061884753.1 2577657..2579006(+) (comFA) [Bacillus thuringiensis strain QZL38]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACGGAAAGAATTAAGTGATTTGAAAAAGAAGGG
AGAAGTCGCATGTGTACAAGGCATAACAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGAT
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAAGATGCACGTATTGCCGGAAGTGCATCACGATGGGGAGAGTTAGT
GAATGTACTGTACTCGTTCGTGGGATTCAGGAAAGAAAGGAAGAAAGAGAGTTAAATCAGTTGCAGTGGAAAGGGGTTTT
GTCTACCGGTCAGGAGTTGGTGGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCTTTCTTTATTTGGGCTGTAT
GCGGTGCTGGAAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGGGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTAGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTGGTTGCGACTACGCATCAGTTATTACGTTATTATAGGGCATTTC
ATGTCATGGTTGTAGATGAAATAGATGCTTTTCCATATTGTGTAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAGAAAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCCTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCCATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAATAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTCGCAGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGTCGAGCAGGGCGGAGCTCTGAAGCGCCGTATGGAGAGGTTATTTATTTTCACTATGGTAAGACAGAAGC
GATGGTACGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGGAAGAACAAGGATTGATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-409)

• DEAD/DEAH box helicase (DEAD) (117-256)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50	98.441	0.492
comFA	Latilactobacillus sakei subsp. sakei 23K	41.791	89.532	0.374