Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VSAL_RS14245 Genome accession   NC_011312
Coordinates   2944289..2944870 (+) Length   193 a.a.
NCBI ID   WP_012551167.1    Uniprot ID   -
Organism   Aliivibrio salmonicida LFI1238     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2939289..2949870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSAL_RS14230 (VSAL_I2715) - 2940107..2942605 (-) 2499 WP_012551164.1 penicillin-binding protein 1A -
  VSAL_RS14235 (VSAL_I2716) pilM 2942744..2943742 (+) 999 WP_012551165.1 type IV pilus biogenesis protein PilM -
  VSAL_RS14240 (VSAL_I2717) - 2943727..2944296 (+) 570 WP_012551166.1 PilN domain-containing protein -
  VSAL_RS14245 (VSAL_I2718) pilO 2944289..2944870 (+) 582 WP_012551167.1 type 4a pilus biogenesis protein PilO Machinery gene
  VSAL_RS14250 (VSAL_I2719) - 2944863..2945381 (+) 519 WP_012551168.1 pilus assembly protein PilP -
  VSAL_RS14255 (VSAL_I2720) - 2945432..2946748 (+) 1317 Protein_2768 type IV pilus secretin PilQ -
  VSAL_RS14260 (VSAL_I2721) - 2946774..2947655 (-) 882 WP_012549007.1 IS982-like element ISVsa6 family transposase -
  VSAL_RS14265 - 2947750..2948136 (+) 387 Protein_2770 type IV pilus secretin PilQ -
  VSAL_RS14270 (VSAL_I2722) aroK 2948361..2948879 (+) 519 WP_012551169.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22708.10 Da        Isoelectric Point: 4.7950

>NTDB_id=31662 VSAL_RS14245 WP_012551167.1 2944289..2944870(+) (pilO) [Aliivibrio salmonicida LFI1238]
MIDWRELDIDEITEWPLLPQLLVVFLLCLCVHAAGYWFWLSPIHEKTEQLKRDELTLKTTIKYKYNQVAAMPEMEDQLNE
LNLRYEFLAQQLPAQKELASMLSGINQVGIQNKLTFTRIDWGEKQNQEFLYKLPLNIELTGQYHDIGSFSEAIADLPRIV
SLENIDIQRVSKESSTLHFRVMAYTYQFRGIHE

Nucleotide


Download         Length: 582 bp        

>NTDB_id=31662 VSAL_RS14245 WP_012551167.1 2944289..2944870(+) (pilO) [Aliivibrio salmonicida LFI1238]
ATGATTGATTGGCGTGAGTTAGATATAGATGAAATTACTGAGTGGCCATTGTTACCTCAGTTACTTGTCGTGTTTTTACT
GTGCCTATGTGTGCATGCCGCAGGGTATTGGTTTTGGTTATCACCGATTCACGAAAAAACAGAGCAATTAAAGAGAGACG
AATTAACGTTAAAAACAACCATCAAATATAAATACAATCAAGTCGCGGCAATGCCTGAAATGGAAGATCAATTAAATGAA
TTGAATTTACGTTATGAATTTTTAGCGCAGCAGCTTCCTGCGCAAAAAGAACTAGCGAGTATGTTGTCAGGGATCAATCA
AGTCGGAATTCAAAACAAACTTACGTTTACTCGTATTGATTGGGGAGAAAAACAGAATCAAGAATTTCTATATAAACTGC
CTCTAAATATCGAACTAACGGGGCAATATCATGATATCGGATCATTTTCAGAAGCAATTGCTGATCTCCCTCGAATTGTA
AGTTTGGAGAATATTGATATTCAACGAGTGAGTAAAGAGAGCAGTACATTGCACTTTCGAGTAATGGCTTATACCTATCA
ATTTAGGGGGATCCATGAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

60.847

97.927

0.596
  pilO Vibrio campbellii strain DS40M4

57.143

97.927

0.56

Multiple sequence alignment