Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   D5H27_RS09350 Genome accession   NZ_CP032506
Coordinates   1968730..1969530 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain JT3-1     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 1963730..1974530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5H27_RS09320 (D5H27_09200) - 1964268..1964900 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  D5H27_RS19775 (D5H27_09205) - 1965197..1965334 (+) 138 WP_014470646.1 hypothetical protein -
  D5H27_RS09330 (D5H27_09210) - 1965428..1966546 (+) 1119 WP_032874152.1 NADH-dependent flavin oxidoreductase -
  D5H27_RS09335 (D5H27_09215) - 1966673..1966885 (+) 213 WP_007408362.1 spore germination protein -
  D5H27_RS09340 (D5H27_09220) - 1967011..1967223 (+) 213 WP_014418351.1 spore germination protein -
  D5H27_RS09345 (D5H27_09225) alr 1967400..1968581 (+) 1182 WP_025649856.1 alanine racemase -
  D5H27_RS09350 (D5H27_09230) spo0A 1968730..1969530 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  D5H27_RS09355 (D5H27_09235) spoIVB 1969811..1971091 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  D5H27_RS09360 (D5H27_09240) recN 1971255..1972985 (-) 1731 WP_032874060.1 DNA repair protein RecN Machinery gene
  D5H27_RS09365 (D5H27_09245) argR 1973017..1973466 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  D5H27_RS09370 (D5H27_09250) - 1973563..1974408 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=316519 D5H27_RS09350 WP_003153177.1 1968730..1969530(-) (spo0A) [Bacillus velezensis strain JT3-1]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=316519 D5H27_RS09350 WP_003153177.1 1968730..1969530(-) (spo0A) [Bacillus velezensis strain JT3-1]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCGTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCAAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGACATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATTGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment