Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   D4A37_RS05860 Genome accession   NZ_CP032365
Coordinates   1130679..1134404 (+) Length   1241 a.a.
NCBI ID   WP_097994407.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain SR52     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1125679..1139404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4A37_RS05845 - 1125697..1126290 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  D4A37_RS05850 lepB 1126487..1127050 (+) 564 WP_048567025.1 signal peptidase I -
  D4A37_RS05855 addB 1127167..1130682 (+) 3516 WP_151035329.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D4A37_RS05860 addA 1130679..1134404 (+) 3726 WP_097994407.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D4A37_RS05865 - 1134417..1134704 (+) 288 WP_001240558.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  D4A37_RS05870 gerPF 1134786..1135001 (-) 216 WP_001141570.1 spore germination protein GerPF -
  D4A37_RS05875 - 1135044..1135430 (-) 387 WP_046958325.1 spore germination protein GerPE -
  D4A37_RS05880 gerPD 1135446..1135640 (-) 195 WP_001052804.1 spore germination protein GerPD -
  D4A37_RS05885 gerPC 1135647..1136261 (-) 615 WP_001070746.1 spore germination protein GerPC -
  D4A37_RS05890 gerPB 1136330..1136536 (-) 207 WP_001012513.1 spore germination protein GerPB -
  D4A37_RS05895 gerPA 1136551..1136772 (-) 222 WP_001111187.1 spore germination protein GerPA -
  D4A37_RS05900 - 1136867..1137046 (-) 180 WP_000462847.1 aspartyl-phosphate phosphatase Spo0E family protein -
  D4A37_RS05905 - 1137293..1138192 (+) 900 WP_174888046.1 fumarylacetoacetate hydrolase family protein -
  D4A37_RS05910 - 1138227..1138511 (-) 285 WP_000926860.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142790.08 Da        Isoelectric Point: 4.8669

>NTDB_id=315185 D4A37_RS05860 WP_097994407.1 1130679..1134404(+) (addA) [Bacillus wiedmannii strain SR52]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAARESWTSVYEAMQNISWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYKYDTNWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=315185 D4A37_RS05860 WP_097994407.1 1130679..1134404(+) (addA) [Bacillus wiedmannii strain SR52]
ATGATTGAAAATTGGCCTGAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATATTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCAGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTCGTGAGTCATGGACAAGCGTTTATGAAGCGATGCAAAACATATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGACATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGTGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGCTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTAGGTGGCGGAATGAAGATAGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTACGATGCAGACGCTGAATTGAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCGGAAGTAGAAAAAGCACAACTTGAAGCTCGCCTTATG
GCGCAGCGAATAAAGGCTATGGTTGATTCAGGATATACCGTCTATGATCGCAAAACGAATGAAATGCGACAAGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGGTTAAATGATGAGGAACTTGCGACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACTGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATAAATATGACACGAACTGGAAA
GTGGAAGTTGTTGACGGCAAAACGTTACTTGCACCGGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAAAGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCT
TTTATTAAAAAACTGCGTGCACCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGATTGACGTACGCAGAGCGTGG
GACAGCAGTCCATGCTGTTATGCAACATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAGCAAATTGCCG
GAATGGTAAATAAGGAACTATTAACATTCGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGATGAAACAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTCAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAGGCGCTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGCAATCATGTTGTCAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1232)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.168

100

0.534

Multiple sequence alignment