Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D4A37_RS00530 Genome accession   NZ_CP032365
Coordinates   97579..98955 (+) Length   458 a.a.
NCBI ID   WP_001085204.1    Uniprot ID   A0A0J7D6B4
Organism   Bacillus wiedmannii strain SR52     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92579..103955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4A37_RS00510 ctsR 92772..93233 (+) 462 WP_001244556.1 transcriptional regulator CtsR -
  D4A37_RS00515 - 93407..93955 (+) 549 WP_000128406.1 UvrB/UvrC motif-containing protein -
  D4A37_RS00520 - 93960..95024 (+) 1065 WP_000050823.1 protein arginine kinase -
  D4A37_RS00525 clpC 95047..97482 (+) 2436 WP_000971181.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  D4A37_RS00530 radA 97579..98955 (+) 1377 WP_001085204.1 DNA repair protein RadA Machinery gene
  D4A37_RS00535 disA 98959..100032 (+) 1074 WP_000392165.1 DNA integrity scanning diadenylate cyclase DisA -
  D4A37_RS00540 - 100193..101296 (+) 1104 WP_000919686.1 PIN/TRAM domain-containing protein -
  D4A37_RS00545 ispD 101313..101993 (+) 681 WP_000288297.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D4A37_RS00550 ispF 102109..102585 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49884.59 Da        Isoelectric Point: 7.4250

>NTDB_id=315177 D4A37_RS00530 WP_001085204.1 97579..98955(+) (radA) [Bacillus wiedmannii strain SR52]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKPTTPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=315177 D4A37_RS00530 WP_001085204.1 97579..98955(+) (radA) [Bacillus wiedmannii strain SR52]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGTTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAATCTGAGGCACGTATTGAAACAAAATTCCAAGAGTTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGATCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACGGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATTCCTATTTTTA
TCGTCGGACATGTAACAAAAGAGGGGGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTCTTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCCGTTGGAGTCGCAG
GATCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATTGCTTCAAGTTTTAGAGATAAACCTACGACACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTCCAACGTGCTATTATTCC
TAGAAAAAATTTGGGGGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTAGGGGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J7D6B4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633

Multiple sequence alignment