Detailed information NTG0314617

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AM490_RS10220
• Genome accession: NZ_CP032257
• Coordinates: 1961700..1962572 (+)
• Length: 290 a.a.
• NCBI ID: WP_003161765.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain AR_0111
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1956700..1967572

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM490_RS10205 (AM490_10205)	-	1958080..1958547 (-)	468	WP_110590994.1	pilin	-
AM490_RS10210 (AM490_10210)	pilB	1958778..1960478 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AM490_RS10215 (AM490_10215)	pilC	1960482..1961699 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
AM490_RS10220 (AM490_10220)	pilD	1961700..1962572 (+)	873	WP_003161765.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
AM490_RS10225 (AM490_10225)	coaE	1962569..1963180 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
AM490_RS10230 (AM490_10230)	yacG	1963177..1963377 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
AM490_RS10235 (AM490_10235)	-	1963414..1963623 (-)	210	WP_020750398.1	hypothetical protein	-
AM490_RS10240 (AM490_10240)	-	1963729..1964418 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
AM490_RS10245 (AM490_10245)	-	1964415..1964885 (-)	471	WP_003094664.1	hypothetical protein	-
AM490_RS10250 (AM490_10250)	-	1964882..1965307 (-)	426	WP_003161766.1	GNAT family N-acetyltransferase	-
AM490_RS10255 (AM490_10255)	-	1965440..1966069 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
AM490_RS10260 (AM490_10260)	-	1966066..1966515 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
AM490_RS10265 (AM490_10265)	-	1966541..1966711 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=314617 AM490_RS10220 WP_003161765.1 1961700..1962572(+) (pilD) [Pseudomonas aeruginosa strain AR_0111]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=314617 AM490_RS10220 WP_003161765.1 1961700..1962572(+) (pilD) [Pseudomonas aeruginosa strain AR_0111]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTTGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469