Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   E4U89_RS06225 Genome accession   NZ_CP038419
Coordinates   1268602..1269339 (-) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli O157:H7 strain 2-6-2     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1263602..1274339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4U89_RS06195 (E4U89_06380) yfiL 1263760..1264125 (-) 366 WP_001301878.1 DUF2799 domain-containing protein -
  E4U89_RS06200 (E4U89_06385) aroF 1264335..1265405 (+) 1071 WP_001168032.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  E4U89_RS06205 (E4U89_06390) tyrA 1265416..1266537 (+) 1122 WP_000225204.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  E4U89_RS06210 (E4U89_06395) pheA 1266580..1267740 (-) 1161 WP_000200120.1 bifunctional chorismate mutase/prephenate dehydratase -
  E4U89_RS06215 (E4U89_06400) pheL 1267839..1267886 (-) 48 WP_001386991.1 pheA operon leader peptide PheL -
  E4U89_RS06220 (E4U89_06405) raiA 1267990..1268331 (-) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  E4U89_RS06225 (E4U89_06410) comL 1268602..1269339 (-) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  E4U89_RS06230 (E4U89_06415) rluD 1269474..1270454 (+) 981 WP_000079092.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  E4U89_RS06235 (E4U89_06420) yfiH 1270451..1271182 (+) 732 WP_000040152.1 purine nucleoside phosphorylase YfiH -
  E4U89_RS06240 (E4U89_06425) clpC 1271312..1273885 (+) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=314478 E4U89_RS06225 WP_000197686.1 1268602..1269339(-) (comL) [Escherichia coli O157:H7 strain 2-6-2]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=314478 E4U89_RS06225 WP_000197686.1 1268602..1269339(-) (comL) [Escherichia coli O157:H7 strain 2-6-2]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCAAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment