Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   D2M30_RS05315 Genome accession   NZ_CP032146
Coordinates   1033718..1037422 (+) Length   1234 a.a.
NCBI ID   WP_125122349.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain YP6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1028718..1042422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS05300 (D2M30_05315) - 1028781..1029308 (-) 528 WP_013351758.1 ferritin-like domain-containing protein -
  D2M30_RS05305 (D2M30_05320) - 1029377..1030090 (-) 714 WP_125122347.1 DUF421 domain-containing protein -
  D2M30_RS05310 (D2M30_05325) addB 1030231..1033731 (+) 3501 WP_125122348.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D2M30_RS05315 (D2M30_05330) addA 1033718..1037422 (+) 3705 WP_125122349.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D2M30_RS05320 (D2M30_05335) sbcD 1037489..1038661 (+) 1173 WP_125122350.1 exonuclease subunit SbcD -
  D2M30_RS05325 (D2M30_05340) sbcC 1038658..1042050 (+) 3393 WP_125122351.1 exonuclease subunit SbcC -
  D2M30_RS05330 (D2M30_05345) - 1042066..1042365 (+) 300 WP_125122352.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141239.79 Da        Isoelectric Point: 5.1275

>NTDB_id=314005 D2M30_RS05315 WP_125122349.1 1033718..1037422(+) (addA) [Bacillus amyloliquefaciens strain YP6]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNVLDDLQQIEELISCRHDFNALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGYEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTEDGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRIFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATVFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQAEQADWLLSEFDRYQASSYLDFIGPALIRHSDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRALIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQDAIDIDELIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=314005 D2M30_RS05315 WP_125122349.1 1033718..1037422(+) (addA) [Bacillus amyloliquefaciens strain YP6]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCGGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTCTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCTAAACAAAAGCTAGAGAAAGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGTGCTGATAATGTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAACGCGCTGTATGAACGGGTTCCTGCGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGCGCTTTTGGACGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTTATTGAAACCCTCGTTCA
GCTCGTAATATCGTACGGAAAACGATTTGAAGCGGCGAAACAGGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTTAAAAGCGGGTA
TGAAGAGGCGGGAAATCTGTTTATGGTGGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGCTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAGATGGGGAAGGTGCCGGGCAGAAAATCGACCTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCGTATCCGCCAAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCCGAAGACTTTGAAACTGTGCATTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTCTATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGCTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCCGTTTACGCCAATTTGA
CGTCAGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTCGCCCTCATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGTGACAGACATGACGACCTGTATCAAAAACTGAGAATAT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAC
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGTCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGTATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTGGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTGAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAACTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGAGCAGGCTGATTGGCTCCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGTCCGGCGCTCATCCGTCACAGTGACATGGAGGCGCACA
GGGCGCCGGGGCTTTTGGCGCCGGAGGATATCGCGCACGACCCGGCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGCCCTGAGGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTCGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAGGAATACGAGGATGAGTACAGCGGACGCGCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACTGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGACGCAATTGATATTGATGAGCTGATTCAGTTTTTCGGCACTGAAATCGGAAAA
GACCTTGTTGGGGCGCTGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTACGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGGATCGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.253

100

0.823

Multiple sequence alignment