Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   D2M30_RS05310 Genome accession   NZ_CP032146
Coordinates   1030231..1033731 (+) Length   1166 a.a.
NCBI ID   WP_125122348.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain YP6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1025231..1038731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS05285 (D2M30_05300) - 1025397..1026554 (-) 1158 WP_071347116.1 AbrB family transcriptional regulator -
  D2M30_RS05290 (D2M30_05305) - 1026719..1027915 (-) 1197 WP_071347115.1 MFS transporter -
  D2M30_RS05295 (D2M30_05310) - 1027931..1028551 (-) 621 WP_125122346.1 TetR/AcrR family transcriptional regulator -
  D2M30_RS05300 (D2M30_05315) - 1028781..1029308 (-) 528 WP_013351758.1 ferritin-like domain-containing protein -
  D2M30_RS05305 (D2M30_05320) - 1029377..1030090 (-) 714 WP_125122347.1 DUF421 domain-containing protein -
  D2M30_RS05310 (D2M30_05325) addB 1030231..1033731 (+) 3501 WP_125122348.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D2M30_RS05315 (D2M30_05330) addA 1033718..1037422 (+) 3705 WP_125122349.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D2M30_RS05320 (D2M30_05335) sbcD 1037489..1038661 (+) 1173 WP_125122350.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133623.21 Da        Isoelectric Point: 5.2384

>NTDB_id=314004 D2M30_RS05310 WP_125122348.1 1030231..1033731(+) (addB) [Bacillus amyloliquefaciens strain YP6]
MGVEFLVGRSGSGKTRLIIDSIQEELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVIKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAVYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDRGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
MAEPLIRLQNRLKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDPERAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYDAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHVHDPMIQASLPMELDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=314004 D2M30_RS05310 WP_125122348.1 1030231..1033731(+) (addB) [Bacillus amyloliquefaciens strain YP6]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGAAGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGCTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCACACGGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATTTGCATTCAGAGGATTATTT
GACCCTGCTCGCTCAGCAGATTCCTTTGGCGGATGTTATAAAAGGCGCCCGTGTTTATGTCGACGGCTTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTCGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCTCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCTCCGGATGAGCTCGATCTGTTCAGAATGACCGGCAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAGTTGAATGCCGATATTTCCTGCAAGGAATTGCATGGAACGAAGCGGCATCAGCATGTGCCGGAGCTTGCCTGTATAG
AATCGCAGTACGATGTGCGTCCCGCGGCTGTGTATACGGGCGGACAGGAAGCCTTTACCGTCATACAGGCACAGAACAGA
AGAGCTGAACTCGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACAGAGGGTACCGGTACAGAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGACTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTTATCCGCTCAAGCTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCCCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAACTACTGTATCGCCTACGGAATCAAAGGTGAGCGCTGGACGAACGGGGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATGGCCGAGCCGCTCATCCGGCTTCAAAACCGGCTGAAAAAAGCGAAAACGGTTCAAAGCATGGCAGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGACAGCAGGCCGAAGAGGCCGGAAACATGATTGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAAGAGTTTGCGGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACCGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTGTCCAGAATGTACGGCACATCCTGCACATTTGTCATCGGAGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTTTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GACATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTCGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAACAGCTCCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTGTGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCCCGAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGAAATGAGGCAAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTTTCCTATGATGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGGCACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTTGGCGGAAGCGGACCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGATAAGGGGCTTGATCTCGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCAATTACACACTCAGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATGTTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGAACTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATTATCAATGCCGGTTT
GAAAAAGGACGGTTCTTTACGGTCCGATTCAGCGGCGGTCAGCGAACGGGATTTTCATCTGCTGACAGACCATGTGAGGC
GGACATTTGAGCAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACTATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.105

100

0.831

Multiple sequence alignment