Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D2M30_RS00585 Genome accession   NZ_CP032146
Coordinates   106609..107988 (+) Length   459 a.a.
NCBI ID   WP_045514053.1    Uniprot ID   A0AAP7N3R0
Organism   Bacillus amyloliquefaciens strain YP6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101609..112988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS00565 (D2M30_00570) ctsR 101957..102421 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  D2M30_RS00570 (D2M30_00575) - 102435..102992 (+) 558 WP_013350789.1 UvrB/UvrC motif-containing protein -
  D2M30_RS00575 (D2M30_00580) - 102992..104083 (+) 1092 WP_013350790.1 protein arginine kinase -
  D2M30_RS00580 (D2M30_00585) clpC 104080..106515 (+) 2436 WP_013350791.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  D2M30_RS00585 (D2M30_00590) radA 106609..107988 (+) 1380 WP_045514053.1 DNA repair protein RadA Machinery gene
  D2M30_RS00590 (D2M30_00595) disA 107992..109074 (+) 1083 WP_014469611.1 DNA integrity scanning diadenylate cyclase DisA -
  D2M30_RS00595 (D2M30_00600) - 109188..110288 (+) 1101 WP_013350794.1 PIN/TRAM domain-containing protein -
  D2M30_RS00600 (D2M30_00605) ispD 110302..111000 (+) 699 WP_071348600.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D2M30_RS00605 (D2M30_00610) ispF 110993..111469 (+) 477 WP_013350796.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49506.91 Da        Isoelectric Point: 7.1316

>NTDB_id=313990 D2M30_RS00585 WP_045514053.1 106609..107988(+) (radA) [Bacillus amyloliquefaciens strain YP6]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=313990 D2M30_RS00585 WP_045514053.1 106609..107988(+) (radA) [Bacillus amyloliquefaciens strain YP6]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGAGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGCATCGGTAAATCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTGCTTTACATTTCTGGGGAAGAATCTGTGAAGCAAA
CGAAGCTGCGTGCCGACAGACTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAGGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAAGGGCTTACAGAAGTGCTGAACCCTTCTGAAATTTTCTTAGAAGAGCGTTCTGCCGGAGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGCACGAGACCGATCTTAGTTGAAATCCAGGCGCTCATATCACCGACCAGCTTC
GGAAATCCGCGCCGCATGGCTACAGGAATTGACCACAACAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTGTTGCTTCAAAATCAAGACGCGTATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCAATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCAAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAGCTCGGCTTCAAGCGTATGGTTATACC
TGAGGCAAATGCAGACGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627

Multiple sequence alignment