Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   D3C60_RS18075 Genome accession   NZ_CP032144
Coordinates   3628231..3628350 (+) Length   39 a.a.
NCBI ID   WP_007407639.1    Uniprot ID   A3KLD5
Organism   Bacillus velezensis strain BIM B-439D     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3623231..3633350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3C60_RS18050 (D3C60_18050) - 3623581..3624093 (+) 513 Protein_3461 bacteriocin biosynthesis protein AlbG -
  D3C60_RS18055 (D3C60_18055) - 3624100..3625476 (-) 1377 WP_032867382.1 YncE family protein -
  D3C60_RS18060 (D3C60_18060) - 3625516..3626871 (-) 1356 WP_032867380.1 YncE family protein -
  D3C60_RS18070 (D3C60_18070) rapF 3627102..3628247 (+) 1146 WP_119001950.1 tetratricopeptide repeat protein Regulator
  D3C60_RS18075 (D3C60_18075) phrF 3628231..3628350 (+) 120 WP_007407639.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  D3C60_RS18080 (D3C60_18080) - 3628548..3628751 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  D3C60_RS18085 (D3C60_18085) - 3628765..3628968 (+) 204 WP_007407641.1 hypothetical protein -
  D3C60_RS18090 (D3C60_18090) - 3629003..3629188 (+) 186 WP_032868172.1 DUF4177 domain-containing protein -
  D3C60_RS18095 (D3C60_18095) - 3629374..3629655 (+) 282 WP_007407643.1 hypothetical protein -
  D3C60_RS18100 (D3C60_18100) - 3629699..3630211 (+) 513 WP_007407644.1 stage II sporulation protein M -
  D3C60_RS18110 (D3C60_18110) - 3630802..3631020 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  D3C60_RS18115 (D3C60_18115) speB 3631188..3632060 (-) 873 WP_007614448.1 agmatinase -
  D3C60_RS18120 (D3C60_18120) speE 3632119..3632949 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4295.19 Da        Isoelectric Point: 10.0233

>NTDB_id=313959 D3C60_RS18075 WP_007407639.1 3628231..3628350(+) (phrF) [Bacillus velezensis strain BIM B-439D]
MKLKYKLALTCLAISTVFVSAEIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=313959 D3C60_RS18075 WP_007407639.1 3628231..3628350(+) (phrF) [Bacillus velezensis strain BIM B-439D]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGAAATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3KLD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462

Multiple sequence alignment