Detailed information NTG0313307

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Ahae11616_RS14645
• Genome accession: NZ_CP032002
• Coordinates: 3040027..3040887 (+)
• Length: 286 a.a.
• NCBI ID: WP_005083855.1
• Uniprot ID: N9F152
• Organism: Acinetobacter haemolyticus strain 11616
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3035027..3045887

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Ahae11616_RS14625 (Ahae11616_14725)	secG	3035551..3035880 (-)	330	WP_004637686.1	preprotein translocase subunit SecG	-
Ahae11616_RS14630 (Ahae11616_14730)	tpiA	3035893..3036684 (-)	792	WP_005088004.1	triose-phosphate isomerase	-
Ahae11616_RS14635 (Ahae11616_14735)	pilB	3037061..3038776 (+)	1716	Protein_2863	type IV-A pilus assembly ATPase PilB	-
Ahae11616_RS14640 (Ahae11616_14740)	pilC	3038801..3040027 (+)	1227	WP_005083853.1	type II secretion system F family protein	Machinery gene
Ahae11616_RS14645 (Ahae11616_14745)	pilD	3040027..3040887 (+)	861	WP_005083855.1	prepilin peptidase	Machinery gene
Ahae11616_RS14650 (Ahae11616_14750)	coaE	3040889..3041494 (+)	606	WP_005088002.1	dephospho-CoA kinase	-
Ahae11616_RS14655 (Ahae11616_14755)	-	3041491..3042402 (-)	912	WP_017394516.1	DMT family transporter	-
Ahae11616_RS14660 (Ahae11616_14765)	rlmB	3042442..3043188 (-)	747	WP_004637678.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
Ahae11616_RS14665 (Ahae11616_14770)	-	3043297..3043623 (-)	327	WP_004637677.1	pyrimidine/purine nucleoside phosphorylase	-
Ahae11616_RS14670 (Ahae11616_14775)	recN	3043957..3045618 (+)	1662	WP_118903225.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32075.38 Da
• Isoelectric Point: 6.6694

>NTDB_id=313307 Ahae11616_RS14645 WP_005083855.1 3040027..3040887(+) (pilD) [Acinetobacter haemolyticus strain 11616]
MQQFLNYFIENPTALYLAVGLFSLCIGSFLNVVIFRTPRMMEQEWQQECQMLLHPEQPIIEEEKLTLSHPASTCPKCHTP
IRWYQNIPVISWLVLRGKCGACQNPISIRYPLIELLTMGCSLVVVAVFGPTLQMLFGLVLTWVLITLTFIDFDTQLLPDR
FTLPLAALGLAINSFAIYTTANSAIWGYVIGFLCLWIVYYLFKIFTGKEGMGYGDFKLLAALGAWMGPMLLPLIVLLSSV
VGAIIGIILLKMRGENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=313307 Ahae11616_RS14645 WP_005083855.1 3040027..3040887(+) (pilD) [Acinetobacter haemolyticus strain 11616]
ATGCAGCAATTCTTAAATTATTTTATCGAAAACCCAACTGCACTTTATCTAGCAGTTGGGCTTTTTAGTTTATGTATTGG
TAGCTTTCTGAATGTTGTTATTTTCCGAACGCCAAGAATGATGGAGCAGGAATGGCAACAAGAATGCCAAATGCTACTCC
ATCCAGAACAACCTATCATTGAAGAAGAAAAGCTGACTTTAAGTCACCCTGCATCAACTTGCCCTAAGTGCCACACCCCT
ATCCGCTGGTATCAAAATATCCCAGTGATCAGCTGGTTAGTACTCCGTGGTAAATGCGGTGCCTGTCAAAACCCGATTAG
TATTCGTTACCCATTGATCGAGTTATTGACCATGGGATGCTCTCTAGTGGTCGTTGCTGTTTTTGGTCCTACCCTCCAAA
TGCTATTCGGTTTAGTTCTGACTTGGGTGTTAATTACCCTTACTTTTATTGATTTTGATACACAATTGCTTCCAGACCGC
TTTACACTCCCATTGGCAGCACTCGGTTTAGCCATTAATAGTTTTGCGATCTACACCACAGCAAACTCAGCAATTTGGGG
TTATGTGATTGGCTTCCTTTGCCTTTGGATTGTTTATTATCTTTTTAAAATATTTACTGGCAAAGAGGGTATGGGCTATG
GCGATTTTAAATTACTGGCTGCATTAGGGGCTTGGATGGGTCCAATGCTCTTGCCTTTAATTGTGTTACTTTCTTCTGTT
GTCGGTGCAATTATTGGTATCATCTTATTGAAAATGCGCGGTGAAAATCAGCCCTTTGCTTTTGGCCCCTATATTGCAAT
TGCAGGCTGGATCGCATTTTTATGGGGTGATCAAATTATGAAAGTTTATTTAGGTGGCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

• Type IV leader peptidase family (Peptidase_A24) (138-247)

3D structure

• AlphaFold DB: N9F152

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	84.965	100	0.85
pilD	Acinetobacter baumannii D1279779	83.916	100	0.839
pilD	Vibrio cholerae strain A1552	55.97	93.706	0.524
pilD	Vibrio campbellii strain DS40M4	54.135	93.007	0.503
pilD	Neisseria gonorrhoeae MS11	47.253	95.455	0.451