Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AhaeAN59_RS14860 Genome accession   NZ_CP031972
Coordinates   3125091..3125951 (+) Length   286 a.a.
NCBI ID   WP_005083855.1    Uniprot ID   N9F152
Organism   Acinetobacter haemolyticus strain AN59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3123782..3124483 3125091..3125951 flank 608


Gene organization within MGE regions


Location: 3123782..3125951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AhaeAN59_RS14850 (AhaeAN59_15005) - 3123782..3124483 (-) 702 WP_001223318.1 IS1-like element ISPa14 family transposase -
  AhaeAN59_RS14855 (AhaeAN59_15010) - 3124543..3125091 (+) 549 Protein_2915 type II secretion system F family protein -
  AhaeAN59_RS14860 (AhaeAN59_15015) pilD 3125091..3125951 (+) 861 WP_005083855.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32075.38 Da        Isoelectric Point: 6.6694

>NTDB_id=313022 AhaeAN59_RS14860 WP_005083855.1 3125091..3125951(+) (pilD) [Acinetobacter haemolyticus strain AN59]
MQQFLNYFIENPTALYLAVGLFSLCIGSFLNVVIFRTPRMMEQEWQQECQMLLHPEQPIIEEEKLTLSHPASTCPKCHTP
IRWYQNIPVISWLVLRGKCGACQNPISIRYPLIELLTMGCSLVVVAVFGPTLQMLFGLVLTWVLITLTFIDFDTQLLPDR
FTLPLAALGLAINSFAIYTTANSAIWGYVIGFLCLWIVYYLFKIFTGKEGMGYGDFKLLAALGAWMGPMLLPLIVLLSSV
VGAIIGIILLKMRGENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=313022 AhaeAN59_RS14860 WP_005083855.1 3125091..3125951(+) (pilD) [Acinetobacter haemolyticus strain AN59]
ATGCAGCAATTCTTAAATTATTTTATCGAAAACCCAACTGCACTTTATCTAGCAGTTGGGCTTTTTAGTTTATGTATTGG
TAGCTTTCTGAATGTTGTTATTTTCCGAACGCCAAGAATGATGGAGCAGGAATGGCAACAAGAATGCCAAATGCTACTCC
ATCCAGAACAACCTATCATTGAAGAAGAAAAGCTGACTTTAAGTCACCCTGCATCAACTTGCCCTAAGTGCCACACCCCT
ATCCGCTGGTATCAAAATATCCCAGTGATCAGCTGGTTAGTACTCCGTGGTAAATGCGGTGCCTGTCAAAACCCGATTAG
TATTCGTTACCCATTGATCGAGTTATTGACCATGGGATGCTCTCTAGTGGTCGTTGCTGTTTTTGGTCCTACCCTCCAAA
TGCTATTCGGTTTAGTTCTGACTTGGGTGTTAATTACCCTTACTTTTATTGATTTTGATACACAATTGCTTCCAGACCGC
TTTACACTCCCATTGGCAGCACTCGGTTTAGCCATTAATAGTTTTGCGATCTACACCACAGCAAACTCAGCAATTTGGGG
TTATGTGATTGGCTTCCTTTGCCTTTGGATTGTTTATTATCTTTTTAAAATATTTACTGGCAAAGAGGGTATGGGCTATG
GCGATTTTAAATTACTGGCTGCATTAGGGGCTTGGATGGGTCCAATGCTCTTGCCTTTAATTGTGTTACTTTCTTCTGTT
GTCGGTGCAATTATTGGTATCATCTTATTGAAAATGCGCGGTGAAAATCAGCCCTTTGCTTTTGGCCCCTATATTGCAAT
TGCAGGCTGGATCGCATTTTTATGGGGTGATCAAATTATGAAAGTTTATTTAGGTGGCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9F152

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

84.965

100

0.85
  pilD Acinetobacter baumannii D1279779

83.916

100

0.839
  pilD Vibrio cholerae strain A1552

55.97

93.706

0.524
  pilD Vibrio campbellii strain DS40M4

54.135

93.007

0.503
  pilD Neisseria gonorrhoeae MS11

47.253

95.455

0.451

Multiple sequence alignment