Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1095_RS06485 Genome accession   NZ_CP031875
Coordinates   1438555..1439103 (-) Length   182 a.a.
NCBI ID   WP_005601803.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 2 str. S1536     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1433555..1444103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1095_RS06470 (D1095_06560) - 1434049..1434570 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  D1095_RS06475 (D1095_06565) - 1434675..1435961 (+) 1287 WP_005601799.1 NAD(P)/FAD-dependent oxidoreductase -
  D1095_RS06480 (D1095_06570) - 1436153..1438480 (-) 2328 WP_005601801.1 Tex family protein -
  D1095_RS06485 (D1095_06575) pilF 1438555..1439103 (-) 549 WP_005601803.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1095_RS06490 (D1095_06580) - 1439157..1440338 (-) 1182 WP_005601805.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1095_RS06510 (D1095_06600) gltX 1441116..1442555 (+) 1440 WP_005601815.1 glutamate--tRNA ligase -
  D1095_RS06520 (D1095_06610) yefM 1442893..1443144 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1095_RS06525 (D1095_06615) - 1443141..1443401 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  D1095_RS06530 (D1095_06620) - 1443560..1443727 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20603.14 Da        Isoelectric Point: 6.6300

>NTDB_id=312291 D1095_RS06485 WP_005601803.1 1438555..1439103(-) (pilF) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=312291 D1095_RS06485 WP_005601803.1 1438555..1439103(-) (pilF) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATACCAACAATTTGAGCAAGCGG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44

Multiple sequence alignment