Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1097_RS06680 Genome accession   NZ_CP031873
Coordinates   1456665..1457213 (-) Length   182 a.a.
NCBI ID   WP_005605049.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 4 str. M62     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1451665..1462213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1097_RS06665 (D1097_06685) - 1452147..1452668 (+) 522 WP_005605042.1 SprT family zinc-dependent metalloprotease -
  D1097_RS06670 (D1097_06690) - 1452773..1454059 (+) 1287 WP_005605045.1 NAD(P)/FAD-dependent oxidoreductase -
  D1097_RS06675 (D1097_06695) - 1454251..1456590 (-) 2340 WP_005605047.1 Tex family protein -
  D1097_RS06680 (D1097_06700) pilF 1456665..1457213 (-) 549 WP_005605049.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1097_RS06685 (D1097_06705) - 1457267..1458448 (-) 1182 WP_005605050.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1097_RS06715 (D1097_06735) gltX 1459476..1460915 (+) 1440 WP_005605060.1 glutamate--tRNA ligase -
  D1097_RS06725 (D1097_06745) yefM 1461253..1461504 (+) 252 WP_005605062.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1097_RS06730 (D1097_06750) - 1461501..1461761 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  D1097_RS06735 (D1097_06755) - 1461920..1462087 (-) 168 WP_005605066.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20689.37 Da        Isoelectric Point: 6.9479

>NTDB_id=312238 D1097_RS06680 WP_005605049.1 1456665..1457213(-) (pilF) [Actinobacillus pleuropneumoniae serovar 4 str. M62]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQITFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSFENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=312238 D1097_RS06680 WP_005605049.1 1456665..1457213(-) (pilF) [Actinobacillus pleuropneumoniae serovar 4 str. M62]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTATTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAATTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGGATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCTTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445

Multiple sequence alignment