Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1111_RS06270 Genome accession   NZ_CP031855
Coordinates   1398772..1399320 (-) Length   182 a.a.
NCBI ID   WP_237593742.1    Uniprot ID   A0A9Q4H8T0
Organism   Actinobacillus pleuropneumoniae strain 7311555     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1393772..1404320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1111_RS06255 (D1111_06305) - 1394254..1394775 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  D1111_RS06260 (D1111_06310) - 1394880..1396166 (+) 1287 WP_005605045.1 NAD(P)/FAD-dependent oxidoreductase -
  D1111_RS06265 (D1111_06315) - 1396358..1398697 (-) 2340 WP_237610206.1 Tex family protein -
  D1111_RS06270 (D1111_06320) pilF 1398772..1399320 (-) 549 WP_237593742.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  D1111_RS06275 (D1111_06325) - 1399374..1400555 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  D1111_RS06300 (D1111_06350) gltX 1401459..1402898 (+) 1440 WP_237610207.1 glutamate--tRNA ligase -
  D1111_RS06310 (D1111_06360) yefM 1403236..1403487 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  D1111_RS06315 (D1111_06365) - 1403484..1403744 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  D1111_RS06320 (D1111_06370) - 1403903..1404070 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20628.24 Da        Isoelectric Point: 6.9477

>NTDB_id=311923 D1111_RS06270 WP_237593742.1 1398772..1399320(-) (pilF) [Actinobacillus pleuropneumoniae strain 7311555]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSNNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=311923 D1111_RS06270 WP_237593742.1 1398772..1399320(-) (pilF) [Actinobacillus pleuropneumoniae strain 7311555]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTACCTACCTCACTCCGTTTTAGCT
TATTATTATCAACAAATCGGTGATCAAGTTCACGCCGAGCAAGCTTATCAGCAAGCATTGAAGCTTAGTAATAACCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

67.553

100

0.698
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445

Multiple sequence alignment