Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   D1F65_RS08090 Genome accession   NZ_CP031779
Coordinates   1621269..1621766 (-) Length   165 a.a.
NCBI ID   WP_117374335.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CFBR-105     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1616269..1626766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1F65_RS08065 (D1F65_08065) gcvPB 1616431..1617903 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  D1F65_RS08070 (D1F65_08070) gcvPA 1617896..1619242 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  D1F65_RS08075 (D1F65_08075) gcvT 1619262..1620353 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  D1F65_RS08080 (D1F65_08080) - 1620512..1621036 (-) 525 WP_001015120.1 shikimate kinase -
  D1F65_RS08085 (D1F65_08085) - 1621026..1621172 (-) 147 WP_001792109.1 hypothetical protein -
  D1F65_RS08090 (D1F65_08090) comGF 1621269..1621766 (-) 498 WP_117374335.1 competence type IV pilus minor pilin ComGF Machinery gene
  D1F65_RS08095 (D1F65_08095) comGE 1621684..1621983 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  D1F65_RS08100 (D1F65_08100) comGD 1621970..1622416 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  D1F65_RS08105 (D1F65_08105) comGC 1622394..1622705 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  D1F65_RS08110 (D1F65_08110) comGB 1622719..1623789 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  D1F65_RS08115 (D1F65_08115) comGA 1623761..1624735 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  D1F65_RS08120 (D1F65_08120) - 1624787..1625410 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  D1F65_RS08125 (D1F65_08125) - 1625407..1625736 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  D1F65_RS08130 (D1F65_08130) - 1625736..1626722 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19123.95 Da        Isoelectric Point: 10.3050

>NTDB_id=311176 D1F65_RS08090 WP_117374335.1 1621269..1621766(-) (comGF) [Staphylococcus aureus strain CFBR-105]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNGRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=311176 D1F65_RS08090 WP_117374335.1 1621269..1621766(-) (comGF) [Staphylococcus aureus strain CFBR-105]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGGCAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

99.394

100

0.994
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment