Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BEN71_RS17250 Genome accession   NZ_CP031716
Coordinates   3470844..3471575 (-) Length   243 a.a.
NCBI ID   WP_068975059.1    Uniprot ID   -
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465844..3476575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS17230 (BEN71_17230) aroB 3466426..3467505 (-) 1080 WP_068975063.1 3-dehydroquinate synthase -
  BEN71_RS17235 (BEN71_17235) aroK 3467537..3468079 (-) 543 WP_068975062.1 shikimate kinase AroK -
  BEN71_RS17240 (BEN71_17240) pilQ 3468116..3470296 (-) 2181 WP_068975061.1 type IV pilus secretin PilQ Machinery gene
  BEN71_RS17245 (BEN71_17245) pilP 3470317..3470847 (-) 531 WP_068975060.1 pilus assembly protein PilP Machinery gene
  BEN71_RS17250 (BEN71_17250) pilO 3470844..3471575 (-) 732 WP_068975059.1 type 4a pilus biogenesis protein PilO Machinery gene
  BEN71_RS17255 (BEN71_17255) pilN 3471572..3472207 (-) 636 WP_068975058.1 PilN domain-containing protein Machinery gene
  BEN71_RS17260 (BEN71_17260) comM 3472207..3473265 (-) 1059 WP_068975057.1 pilus assembly protein PilM Machinery gene
  BEN71_RS17265 (BEN71_17265) ponA 3473430..3475985 (+) 2556 WP_068975056.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 243 a.a.        Molecular weight: 27427.00 Da        Isoelectric Point: 4.8229

>NTDB_id=310699 BEN71_RS17250 WP_068975059.1 3470844..3471575(-) (pilO) [Acinetobacter wuhouensis strain WCHA60]
MSREDFEQLDSSVDAPKKNKMTVEKFFQQFNTLDMNNYGSWPWSVKITCWLFLFFVVCAIGYFLAIKPKLDAIDQARTVE
QNLLNEFKEKDSKLRNLQQYQMQLQQMEANFNQQLEQLPKETEIPSLVEDINVTGVSSGLKFKNIRLEQEVKQEFFIEQP
ISIDATGDYHSFGSFVSSIAALPRIVTLHDFTISGVPSQDKKSDVPVVDYTIKAKTYRYVGADGQGAGNTNASATPPQGG
AAQ

Nucleotide


Download         Length: 732 bp        

>NTDB_id=310699 BEN71_RS17250 WP_068975059.1 3470844..3471575(-) (pilO) [Acinetobacter wuhouensis strain WCHA60]
ATGAGTCGTGAAGATTTTGAGCAATTAGATTCATCTGTTGATGCTCCAAAGAAAAATAAAATGACCGTTGAGAAGTTTTT
TCAACAGTTTAATACGCTGGATATGAATAATTATGGTAGCTGGCCGTGGTCGGTTAAGATTACTTGCTGGTTATTCTTAT
TTTTTGTGGTTTGTGCAATAGGTTACTTTTTAGCAATAAAGCCGAAACTTGACGCGATTGATCAAGCCCGTACAGTTGAA
CAAAATTTGCTGAATGAATTTAAAGAAAAAGATTCTAAATTACGTAATTTGCAACAATATCAAATGCAACTACAGCAAAT
GGAAGCGAATTTTAATCAGCAACTAGAGCAATTGCCGAAAGAAACAGAAATTCCAAGTCTAGTTGAAGATATTAACGTGA
CAGGTGTCAGTTCAGGTCTGAAATTTAAAAATATTAGATTGGAACAAGAAGTTAAGCAAGAGTTCTTTATTGAACAACCG
ATCTCGATTGATGCGACTGGTGATTATCATTCATTTGGTAGTTTTGTCAGTAGTATTGCTGCTTTACCTCGTATTGTAAC
TTTGCATGACTTCACAATTTCAGGTGTGCCGAGCCAAGATAAAAAATCAGATGTGCCTGTTGTAGATTATACAATTAAAG
CAAAAACTTACCGTTATGTTGGTGCTGATGGACAAGGGGCAGGTAATACCAATGCCTCAGCGACACCACCGCAAGGAGGG
GCGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

73.874

91.358

0.675
  comO Acinetobacter baylyi ADP1

70.742

94.239

0.667

Multiple sequence alignment