Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH597_RS03535 Genome accession   NZ_CP031688
Coordinates   689517..690056 (-) Length   179 a.a.
NCBI ID   WP_070828220.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P636-8296     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 684517..695056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH597_RS03505 (CH597_03500) fdx 684920..685261 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH597_RS03510 (CH597_03505) iscX 685261..685455 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  CH597_RS03515 (CH597_03510) - 685506..686120 (-) 615 WP_011272010.1 YfgM family protein -
  CH597_RS03520 (CH597_03515) hisS 686138..687409 (-) 1272 WP_038440737.1 histidine--tRNA ligase -
  CH597_RS03525 (CH597_03520) ispG 687419..688525 (-) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH597_RS03530 (CH597_03525) - 688534..689445 (-) 912 WP_042611894.1 RodZ domain-containing protein -
  CH597_RS03535 (CH597_03530) pilF/pilF2 689517..690056 (-) 540 WP_070828220.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH597_RS03540 (CH597_03535) - 690157..691308 (-) 1152 WP_070828221.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH597_RS03545 (CH597_03540) - 691554..692432 (+) 879 WP_070828222.1 D-alanyl-D-alanine carboxypeptidase family protein -
  CH597_RS03550 (CH597_03545) - 692525..692989 (-) 465 WP_070828223.1 hypothetical protein -
  CH597_RS03555 (CH597_03550) - 693160..694041 (+) 882 WP_050838496.1 metal ABC transporter substrate-binding protein -
  CH597_RS03560 (CH597_03555) - 694041..694961 (+) 921 WP_048954967.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20636.38 Da        Isoelectric Point: 8.6194

>NTDB_id=310128 CH597_RS03535 WP_070828220.1 689517..690056(-) (pilF/pilF2) [Haemophilus influenzae strain P636-8296]
MQTIIKQLSAVIFPFIFSACVSQSASNLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=310128 CH597_RS03535 WP_070828220.1 689517..690056(-) (pilF/pilF2) [Haemophilus influenzae strain P636-8296]
ATGCAAACAATAATTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAGATCATCAAGCTGCCGCCAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(73-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.089

100

0.961
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment