Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH611_RS03255 Genome accession   NZ_CP031685
Coordinates   641314..641853 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain P650-8603     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636314..646853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH611_RS03225 (CH611_03225) fdx 636717..637058 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH611_RS03230 (CH611_03230) iscX 637058..637252 (+) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  CH611_RS03235 (CH611_03235) - 637303..637917 (-) 615 WP_116945256.1 YfgM family protein -
  CH611_RS03240 (CH611_03240) hisS 637935..639206 (-) 1272 WP_116945255.1 histidine--tRNA ligase -
  CH611_RS03245 (CH611_03245) ispG 639216..640322 (-) 1107 WP_005649145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH611_RS03250 (CH611_03250) - 640331..641242 (-) 912 WP_005691759.1 RodZ family helix-turn-helix domain-containing protein -
  CH611_RS03255 (CH611_03255) pilF/pilF2 641314..641853 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH611_RS03260 (CH611_03260) - 641954..643105 (-) 1152 WP_050398522.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH611_RS03265 (CH611_03265) - 643352..644230 (+) 879 WP_005691763.1 serine hydrolase -
  CH611_RS09405 - 644222..644347 (-) 126 WP_257005528.1 hypothetical protein -
  CH611_RS03270 (CH611_03270) - 644595..645476 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  CH611_RS03275 (CH611_03275) - 645476..646396 (+) 921 WP_005662663.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=310024 CH611_RS03255 WP_005654843.1 641314..641853(-) (pilF/pilF2) [Haemophilus influenzae strain P650-8603]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=310024 CH611_RS03255 WP_005654843.1 641314..641853(-) (pilF/pilF2) [Haemophilus influenzae strain P650-8603]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment