Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH621_RS03685 Genome accession   NZ_CP031683
Coordinates   713921..714460 (-) Length   179 a.a.
NCBI ID   WP_105872402.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P662-7189     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708921..719460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH621_RS03655 (CH621_03655) fdx 709327..709668 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH621_RS03660 (CH621_03660) iscX 709668..709862 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  CH621_RS03665 (CH621_03665) - 709910..710524 (-) 615 WP_105872406.1 YfgM family protein -
  CH621_RS03670 (CH621_03670) hisS 710542..711813 (-) 1272 WP_105872405.1 histidine--tRNA ligase -
  CH621_RS03675 (CH621_03675) ispG 711823..712929 (-) 1107 WP_105872404.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH621_RS03680 (CH621_03680) - 712938..713849 (-) 912 WP_105872403.1 RodZ family helix-turn-helix domain-containing protein -
  CH621_RS03685 (CH621_03685) pilF/pilF2 713921..714460 (-) 540 WP_105872402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH621_RS03690 (CH621_03690) - 714561..715712 (-) 1152 WP_050838499.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH621_RS03695 (CH621_03695) - 715958..716836 (+) 879 WP_011961721.1 serine hydrolase -
  CH621_RS03700 (CH621_03700) - 716930..717394 (-) 465 WP_011961722.1 hypothetical protein -
  CH621_RS03705 (CH621_03705) - 717722..718582 (+) 861 WP_224064323.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20661.48 Da        Isoelectric Point: 9.4145

>NTDB_id=309953 CH621_RS03685 WP_105872402.1 713921..714460(-) (pilF/pilF2) [Haemophilus influenzae strain P662-7189]
MQTIIKQLSAVIFPFIFSACVSQSVSNLDHQAAAKARVELALNYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYKIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=309953 CH621_RS03685 WP_105872402.1 713921..714460(-) (pilF/pilF2) [Haemophilus influenzae strain P662-7189]
ATGCAAACAATAATTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGTATCTAA
CTTAGATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTTGCATTAAACTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATAAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACATTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.296

100

0.933
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment