Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH624_RS03715 Genome accession   NZ_CP031682
Coordinates   709760..710299 (-) Length   179 a.a.
NCBI ID   WP_112109780.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P665-7858     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704760..715299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH624_RS03685 (CH624_03685) fdx 705163..705504 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH624_RS03690 (CH624_03690) iscX 705504..705698 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  CH624_RS03695 (CH624_03695) - 705749..706363 (-) 615 WP_112109782.1 YfgM family protein -
  CH624_RS03700 (CH624_03700) hisS 706381..707652 (-) 1272 WP_042611740.1 histidine--tRNA ligase -
  CH624_RS03705 (CH624_03705) ispG 707662..708768 (-) 1107 WP_005649145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH624_RS03710 (CH624_03710) - 708777..709688 (-) 912 WP_112109781.1 RodZ domain-containing protein -
  CH624_RS03715 (CH624_03715) pilF/pilF2 709760..710299 (-) 540 WP_112109780.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH624_RS03720 (CH624_03720) - 710400..711551 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH624_RS03725 (CH624_03725) - 711798..712676 (+) 879 WP_005691763.1 D-alanyl-D-alanine carboxypeptidase family protein -
  CH624_RS03730 (CH624_03730) - 713042..713923 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  CH624_RS03735 (CH624_03735) - 713923..714843 (+) 921 WP_005662663.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.21 Da        Isoelectric Point: 8.2690

>NTDB_id=309920 CH624_RS03715 WP_112109780.1 709760..710299(-) (pilF/pilF2) [Haemophilus influenzae strain P665-7858]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLTKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAHQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=309920 CH624_RS03715 WP_112109780.1 709760..710299(-) (pilF/pilF2) [Haemophilus influenzae strain P665-7858]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGACTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCATCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCACAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

44.134

100

0.441

Multiple sequence alignment