Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CH631_RS03345 Genome accession   NZ_CP031680
Coordinates   666881..667420 (-) Length   179 a.a.
NCBI ID   WP_075913234.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P672-7661     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661881..672420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH631_RS03315 (CH631_03315) fdx 662284..662625 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  CH631_RS03320 (CH631_03320) iscX 662625..662819 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  CH631_RS03325 (CH631_03325) - 662870..663484 (-) 615 WP_105876738.1 YfgM family protein -
  CH631_RS03330 (CH631_03330) hisS 663502..664773 (-) 1272 WP_013527477.1 histidine--tRNA ligase -
  CH631_RS03335 (CH631_03335) ispG 664783..665889 (-) 1107 WP_112122554.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CH631_RS03340 (CH631_03340) - 665898..666809 (-) 912 WP_112122553.1 RodZ domain-containing protein -
  CH631_RS03345 (CH631_03345) pilF/pilF2 666881..667420 (-) 540 WP_075913234.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CH631_RS03350 (CH631_03350) - 667521..668672 (-) 1152 WP_065251304.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CH631_RS03355 (CH631_03355) - 668919..669797 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  CH631_RS03360 (CH631_03360) - 670163..671044 (+) 882 WP_112119364.1 metal ABC transporter substrate-binding protein -
  CH631_RS03365 (CH631_03365) - 671044..671964 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20568.22 Da        Isoelectric Point: 8.6216

>NTDB_id=309850 CH631_RS03345 WP_075913234.1 666881..667420(-) (pilF/pilF2) [Haemophilus influenzae strain P672-7661]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=309850 CH631_RS03345 WP_075913234.1 666881..667420(-) (pilF/pilF2) [Haemophilus influenzae strain P672-7661]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

99.441

100

0.994
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment