Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DD547_RS08765 Genome accession   NZ_CP031670
Coordinates   1691022..1691519 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 64     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686022..1696519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DD547_RS08740 (DD547_01622) gcvPB 1686184..1687656 (-) 1473 WP_050956458.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DD547_RS08745 (DD547_01623) gcvPA 1687649..1688995 (-) 1347 WP_048665334.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DD547_RS08750 (DD547_01624) gcvT 1689015..1690106 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DD547_RS08755 (DD547_01625) - 1690265..1690789 (-) 525 WP_001015117.1 shikimate kinase -
  DD547_RS08760 - 1690779..1690925 (-) 147 WP_001789879.1 hypothetical protein -
  DD547_RS08765 (DD547_01626) comGF 1691022..1691519 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  DD547_RS08770 (DD547_01627) comGE 1691437..1691736 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DD547_RS08775 (DD547_01628) comGD 1691723..1692169 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  DD547_RS08780 (DD547_01629) comGC 1692147..1692458 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DD547_RS08785 (DD547_01630) comGB 1692472..1693542 (-) 1071 Protein_1662 competence type IV pilus assembly protein ComGB -
  DD547_RS08790 (DD547_01631) comGA 1693514..1694488 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  DD547_RS08795 (DD547_01632) - 1694540..1695163 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DD547_RS08800 (DD547_01633) - 1695160..1695489 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DD547_RS08805 (DD547_01634) - 1695489..1696475 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=309625 DD547_RS08765 WP_001789864.1 1691022..1691519(-) (comGF) [Staphylococcus aureus strain 64]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=309625 DD547_RS08765 WP_001789864.1 1691022..1691519(-) (comGF) [Staphylococcus aureus strain 64]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment