Detailed information
Bioinformatically predicted
Overview
| Name | pilA | Type | Machinery gene |
| Locus tag | EKI55_RS07255 | Genome accession | NZ_CP037941 |
| Coordinates | 1488016..1488456 (-) | Length | 146 a.a. |
| NCBI ID | WP_000360902.1 | Uniprot ID | A7ZHJ7 |
| Organism | Escherichia coli strain CFSAN027350 | ||
| Function | type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake |
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Genomic Context
Location: 1483016..1493456
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKI55_RS07235 (EKI55_07235) | coaE | 1483506..1484126 (-) | 621 | WP_001269520.1 | dephospho-CoA kinase | - |
| EKI55_RS28975 | - | 1484151..1484195 (+) | 45 | WP_120795372.1 | protein YacM | - |
| EKI55_RS07240 (EKI55_07240) | guaC | 1484351..1485394 (+) | 1044 | WP_001217338.1 | GMP reductase | - |
| EKI55_RS07245 (EKI55_07245) | hofC | 1485429..1486631 (-) | 1203 | WP_000157234.1 | protein transport protein HofC | - |
| EKI55_RS07250 (EKI55_07250) | pilB | 1486621..1488006 (-) | 1386 | WP_001025155.1 | type II secretion system protein GspE | Machinery gene |
| EKI55_RS07255 (EKI55_07255) | pilA | 1488016..1488456 (-) | 441 | WP_000360902.1 | prepilin peptidase-dependent pilin | Machinery gene |
| EKI55_RS07260 (EKI55_07260) | nadC | 1488659..1489552 (-) | 894 | WP_001135174.1 | carboxylating nicotinate-nucleotide diphosphorylase | - |
| EKI55_RS07265 (EKI55_07265) | ampD | 1489640..1490191 (+) | 552 | WP_000923721.1 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD | - |
| EKI55_RS07270 (EKI55_07270) | ampE | 1490188..1491042 (+) | 855 | WP_000172006.1 | beta-lactamase regulator AmpE | - |
| EKI55_RS07275 (EKI55_07275) | aroP | 1491085..1492458 (-) | 1374 | WP_012817732.1 | aromatic amino acid transporter AroP | - |
Sequence
Protein
Download Length: 146 a.a. Molecular weight: 15630.76 Da Isoelectric Point: 4.5559
>NTDB_id=308960 EKI55_RS07255 WP_000360902.1 1488016..1488456(-) (pilA) [Escherichia coli strain CFSAN027350]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN
Nucleotide
Download Length: 441 bp
>NTDB_id=308960 EKI55_RS07255 WP_000360902.1 1488016..1488456(-) (pilA) [Escherichia coli strain CFSAN027350]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA | Haemophilus influenzae 86-028NP |
41.791 |
91.781 |
0.384 |
| pilA | Haemophilus influenzae Rd KW20 |
41.045 |
91.781 |
0.377 |