Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BPHYT_RS02905 Genome accession   NC_010681
Coordinates   646392..647903 (-) Length   503 a.a.
NCBI ID   WP_012431659.1    Uniprot ID   B2SXH6
Organism   Paraburkholderia phytofirmans PsJN     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641392..652903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPHYT_RS02880 (Bphyt_0591) - 642310..642945 (-) 636 WP_012431654.1 LON peptidase substrate-binding domain-containing protein -
  BPHYT_RS02885 (Bphyt_0592) rapZ 642985..643878 (-) 894 WP_012431655.1 RNase adapter RapZ -
  BPHYT_RS02890 (Bphyt_0593) hprK 643976..644944 (-) 969 WP_012431656.1 HPr(Ser) kinase/phosphatase -
  BPHYT_RS02895 (Bphyt_0594) - 645072..645587 (-) 516 WP_012431657.1 PTS sugar transporter subunit IIA -
  BPHYT_RS02900 (Bphyt_0595) hpf 645865..646221 (-) 357 WP_012431658.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BPHYT_RS02905 (Bphyt_0596) rpoN/rpoN1 646392..647903 (-) 1512 WP_012431659.1 RNA polymerase factor sigma-54 Machinery gene
  BPHYT_RS02910 (Bphyt_0597) lptB 648091..648876 (-) 786 WP_012431660.1 LPS export ABC transporter ATP-binding protein -
  BPHYT_RS02915 (Bphyt_0598) lptA 648873..649544 (-) 672 WP_012431661.1 lipopolysaccharide transport periplasmic protein LptA -
  BPHYT_RS02920 (Bphyt_0599) lptC 649565..650173 (-) 609 WP_012431662.1 LPS export ABC transporter periplasmic protein LptC -
  BPHYT_RS02925 (Bphyt_0600) - 650170..650730 (-) 561 WP_012431663.1 HAD family hydrolase -
  BPHYT_RS02930 (Bphyt_0601) kdsD 650730..651713 (-) 984 WP_012431664.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55248.39 Da        Isoelectric Point: 5.0706

>NTDB_id=30854 BPHYT_RS02905 WP_012431659.1 646392..647903(-) (rpoN/rpoN1) [Paraburkholderia phytofirmans PsJN]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSAPPDQM
GGNTSSSSSSERAENGEPQGVDEYNGLAGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVTQA
GQRDRALITFLIESLDDDGYLAATLEEVLADLPEELEVDLDEMNAALALLHSFDPAGVGARSASECLKLQLLRLDASPTR
TLALDIVAHHLELLAARDFTRLRKHLKANDDDLRDAHILIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNPEV
VPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=30854 BPHYT_RS02905 WP_012431659.1 646392..647903(-) (rpoN/rpoN1) [Paraburkholderia phytofirmans PsJN]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACGCCGCAACTGCAGCAGTCCATCCGGCTGCTACA
GCTGTCTACGCTCGAATTGCAGCAAGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGTTCGCTGATCGCGCAGGCGCCCAACTCTTCCGCGCCGCCCGACCAGATG
GGCGGCAATACGTCGTCCTCGTCCAGCAGCGAGCGCGCCGAGAACGGCGAGCCGCAGGGCGTGGACGAATACAACGGCCT
CGCGGGCGACGGCAATGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCGGACGACGACG
ACCTGCCGCCGCTGCAAATCCATGAATCGAGCACCTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTCACCCAGGCG
GGCCAGCGCGACCGTGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGGCCGCCACGCTCGAAGA
AGTGCTGGCCGATCTGCCCGAAGAGCTCGAAGTCGACCTCGACGAAATGAACGCCGCGCTCGCGCTGCTGCATAGCTTCG
ATCCGGCCGGAGTGGGTGCGCGCTCCGCGTCGGAATGTCTCAAGCTGCAATTGCTGCGACTCGATGCCTCGCCTACACGC
ACGCTCGCGCTAGACATCGTCGCCCATCATCTGGAACTGCTCGCGGCACGCGATTTCACGCGTCTGCGCAAGCATCTGAA
GGCCAACGACGACGATCTGCGCGACGCGCACATACTGATCCGCTCGCTCGAACCGTTCCCCGGCGCCGCCTATGGCAAGG
CCGAAGCGGACTATGTCGTACCGGACATCATGGTGCGCAAAACAGCACAGGGCTGGCAGGCCGAGCTGAATCCGGAAGTG
GTGCCGAAGCTGCGTATTAACCATCTCTACGCGAACATTCTGCGCAATAACCGGGGCGATCCGGGCAGCGGTTCGTTGCG
CCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGAGACGATTCTGCGCGTGGCGCAGGCAA
TTGTGGAACGTCAGAAGAGCTTTTTTGTCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCTGATACG
CTGGGCCTACACGAGTCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGAATTTAA
GTACTTCTTCGGATCACACGTTTCGACTGACACGGGCGGCGCGGCGTCTTCAACGGCGATTCGCGCGCTTATCAAGCAAC
TGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCGGACAGTCGCATAGCCGAACTGCTGGCAGAACAGGGCTTCGTGGTC
GCACGGCGTACCGTTGCGAAATACCGTGAAGCGCTCAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2SXH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.405

100

0.672

Multiple sequence alignment