Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   DRC71_RS01100 Genome accession   NZ_CP031605
Coordinates   238294..239643 (-) Length   449 a.a.
NCBI ID   WP_140239106.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain VB16     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 217556..273741 238294..239643 within 0


Gene organization within MGE regions


Location: 217556..273741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DRC71_RS00990 (DRC71_01015) mecA 217556..219565 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  DRC71_RS00995 (DRC71_01020) mecR1 219662..221419 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  DRC71_RS01000 (DRC71_01025) mecI 221419..221790 (+) 372 WP_025904161.1 mecA-type methicillin resistance repressor MecI -
  DRC71_RS01005 (DRC71_01030) psm-mec 221875..221943 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  DRC71_RS01010 (DRC71_01035) - 222263..223411 (+) 1149 WP_075773656.1 ROK family transcriptional regulator -
  DRC71_RS01015 (DRC71_01045) cstB 223525..224859 (-) 1335 Protein_198 persulfide dioxygenase-sulfurtransferase CstB -
  DRC71_RS01020 (DRC71_01050) - 224892..225956 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  DRC71_RS01025 (DRC71_01055) cstR 226092..226352 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  DRC71_RS01030 (DRC71_01060) - 226352..226990 (+) 639 Protein_201 sulfite exporter TauE/SafE family protein -
  DRC71_RS01040 (DRC71_01070) - 227264..227881 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  DRC71_RS01045 (DRC71_01075) - 227962..230376 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  DRC71_RS01050 (DRC71_01080) - 230369..230734 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  DRC71_RS01055 (DRC71_01085) - 230972..231349 (-) 378 WP_140239103.1 DUF6262 family protein -
  DRC71_RS01060 (DRC71_01090) - 231356..233248 (-) 1893 WP_002485298.1 site-specific integrase -
  DRC71_RS01065 (DRC71_01095) - 233443..233766 (-) 324 WP_000088091.1 JAB domain-containing protein -
  DRC71_RS01070 (DRC71_01100) - 233759..233964 (-) 206 Protein_208 hypothetical protein -
  DRC71_RS01075 (DRC71_01105) - 233966..234487 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  DRC71_RS01080 (DRC71_01110) - 234506..234817 (-) 312 WP_000833212.1 DUF960 family protein -
  DRC71_RS01085 (DRC71_01120) - 234902..235252 (-) 351 WP_140239104.1 SAUGI family uracil-DNA glycosylase inhibitor -
  DRC71_RS01090 (DRC71_01125) - 235823..236557 (-) 735 WP_075100305.1 glucose 1-dehydrogenase -
  DRC71_RS01095 (DRC71_01130) ccrB 236645..238273 (-) 1629 WP_140239105.1 cassette chromosome recombinase CcrB Machinery gene
  DRC71_RS01100 (DRC71_01135) ccrA 238294..239643 (-) 1350 WP_140239106.1 cassette chromosome recombinase CcrA Machinery gene
  DRC71_RS01105 (DRC71_01140) - 239821..240078 (-) 258 WP_058332293.1 hypothetical protein -
  DRC71_RS01110 (DRC71_01145) cch1 240141..241931 (-) 1791 WP_189695518.1 cassette chromosome replicative helicase -
  DRC71_RS01115 (DRC71_01150) - 242057..243484 (-) 1428 WP_140239108.1 DUF6038 family protein -
  DRC71_RS01120 (DRC71_01155) - 243701..244864 (+) 1164 WP_103388710.1 ATP-binding protein -
  DRC71_RS01125 (DRC71_01160) - 245010..245666 (+) 657 WP_257976475.1 hypothetical protein -
  DRC71_RS01130 (DRC71_01165) - 245803..246428 (+) 626 Protein_220 type I restriction endonuclease -
  DRC71_RS13645 - 246452..247222 (+) 771 WP_257976476.1 HNH endonuclease -
  DRC71_RS01140 - 247519..247701 (-) 183 WP_189695519.1 hypothetical protein -
  DRC71_RS01145 (DRC71_01175) - 248888..249532 (+) 645 WP_206665915.1 hypothetical protein -
  DRC71_RS01150 (DRC71_01180) - 249582..250586 (-) 1005 WP_140239109.1 hypothetical protein -
  DRC71_RS01155 (DRC71_01185) - 250615..251940 (-) 1326 WP_140239110.1 TcaA second domain-containing protein -
  DRC71_RS01160 (DRC71_01190) - 251963..252280 (-) 318 WP_140239111.1 type II toxin-antitoxin system PemK/MazF family toxin -
  DRC71_RS01165 (DRC71_01195) - 252283..252531 (-) 249 WP_140239112.1 AbrB family transcriptional regulator -
  DRC71_RS01170 (DRC71_01200) - 252647..253726 (+) 1080 WP_140239113.1 AbiH family protein -
  DRC71_RS13650 - 253905..253994 (-) 90 Protein_229 putative holin-like toxin -
  DRC71_RS01175 (DRC71_01210) - 255723..257543 (+) 1821 WP_189695520.1 DNA/RNA helicase domain-containing protein -
  DRC71_RS01180 (DRC71_01215) - 257753..260545 (+) 2793 WP_140239115.1 type I restriction endonuclease subunit R -
  DRC71_RS01185 (DRC71_01220) - 260635..261216 (+) 582 WP_140239116.1 restriction endonuclease subunit S -
  DRC71_RS01190 (DRC71_01225) - 261217..262773 (+) 1557 WP_189695521.1 type I restriction-modification system subunit M -
  DRC71_RS13655 (DRC71_01230) - 262766..263989 (+) 1224 WP_140239118.1 restriction endonuclease subunit S -
  DRC71_RS01200 (DRC71_01235) - 264038..264286 (+) 249 WP_140239119.1 hypothetical protein -
  DRC71_RS01205 - 264350..264571 (-) 222 WP_180932475.1 hypothetical protein -
  DRC71_RS01210 (DRC71_01240) - 264586..265089 (-) 504 WP_140239120.1 DUF1643 domain-containing protein -
  DRC71_RS01215 (DRC71_01245) - 265104..265415 (-) 312 WP_031912426.1 DUF960 family protein -
  DRC71_RS01220 (DRC71_01250) - 265511..265849 (-) 339 WP_069995843.1 SAUGI family uracil-DNA glycosylase inhibitor -
  DRC71_RS01225 (DRC71_01255) ccrB 266318..267946 (-) 1629 WP_031912428.1 cassette chromosome recombinase CcrB Machinery gene
  DRC71_RS01230 (DRC71_01260) ccrA 267968..269317 (-) 1350 WP_140239121.1 cassette chromosome recombinase CcrA Machinery gene
  DRC71_RS01235 (DRC71_01265) - 269506..270135 (-) 630 Protein_242 hypothetical protein -
  DRC71_RS13660 (DRC71_01270) - 270144..270482 (+) 339 Protein_243 recombinase family protein -
  DRC71_RS01240 (DRC71_01275) - 270594..270953 (-) 360 WP_001631039.1 hypothetical protein -
  DRC71_RS01245 (DRC71_01280) - 271160..271486 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  DRC71_RS01250 (DRC71_01290) - 271816..273741 (+) 1926 WP_017175150.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52209.97 Da        Isoelectric Point: 10.0278

>NTDB_id=307848 DRC71_RS01100 WP_140239106.1 238294..239643(-) (ccrA) [Staphylococcus pseudintermedius strain VB16]
MKQAIGYLRQSTTKQQSLAAQKQTIKVLAEKHNIQHITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKLITTHAPFGYHYH
NGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKLINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTI
YEEAQVTRTQKQVKRKPSENQLKQKIKCPYCHSTLTNITVRKTDHSLRYYVCPQNMNNARFVCEFKGINAQELETSVLAT
CQDFFQNQQLYSKINHTIRQRLKRQRMHDTSNTLTQEKLIENLAQGKIDVETFREQSQSINLQNKPIQAISDIRIKASLQ
KVIQKSFTLNMLNPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=307848 DRC71_RS01100 WP_140239106.1 238294..239643(-) (ccrA) [Staphylococcus pseudintermedius strain VB16]
ATGAAACAAGCAATTGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCACAAAAACAAACCATCAAGGT
ATTAGCCGAAAAACATAATATTCAACACATTACCTTTTATAGCGATAAACAATCAGGACGTACTGATAAGCGGAATGGTT
ATCAACAAATTACTGAACTAATTCAACAGGGACAATGTGACGTATTATGTTGTTATAGATTAAACCGACTTCATCGCAAT
CTTAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAATATCATGTTCATATCTTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCTGAACTAGAATCTGATAATATAGGTGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAACTGATTACAACACATGCACCCTTTGGGTATCACTATCAT
AATGGTACATTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTATTACCTTCAAGGTTATGGTTA
TAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAACTCATTAATCGCAAGCCCTATCAAGTGCGTAATATTATCCTTA
ACCCTAATTACTGTGGCCGTGTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACAAGTCAAACGTAAGCCGTCAGAAAATCAACTCAAACAAAAAATCAA
ATGTCCCTATTGTCATTCGACACTCACAAACATAACTGTCCGAAAAACAGATCATTCATTGCGTTACTATGTCTGTCCGC
AAAATATGAATAATGCTCGCTTTGTTTGTGAATTTAAAGGAATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCGTCAACGACTCAAAAGACAAAGGAT
GCATGATACTAGTAACACATTAACTCAGGAAAAGCTTATTGAAAATTTAGCACAAGGAAAAATTGATGTAGAAACATTCA
GAGAACAGTCCCAATCAATTAATCTACAGAATAAACCTATACAAGCAATAAGTGATATTCGTATAAAAGCATCACTACAA
AAGGTTATACAGAAAAGTTTCACGTTAAACATGCTGAATCCCTATATTGATGAAATTCGCATTACAAAAAACAAAGCCCT
TGTTGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGTTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(170-249)

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

87.973

100

0.88
  ccrA Staphylococcus aureus N315

72.606

100

0.726

Multiple sequence alignment